Chemical and antibacterial analysis of Cinnamomum verum leaves extract and fractions against multidrug resistant bacteria

Abstract

The chemical composition, the antioxidant and antimicrobial potential of crude extract from leaves Cinnamomum verum and their enriched fractions was studied. Phytochemical analyses were performed by TLC and HPLC, and the antioxidant capacity was verified by DPPH^• and ABTS^•+. The Minimal Inhibitory/Bactericidal Concentration was conducted against twenty-two bacteria to select five strains susceptible to extracts/fractions and resistant to the antibiotics tested. Interference of Ethyl Acetate Fraction (EAF) in resistance to synthetic antibiotic was assayed by modulatory and checkerboard model. The chromatographic data showed phenolic compounds in crude extract, as well the flavonoid enrichment in the EAF. The combination of EAF and synthetic antibiotics (ampicillin, azithromycin, ciprofloxacin, or gentamicin) provides a synergistic effect against multidrug resistant strains). The results are useful to obtain multi-targeting in a single therapy solution, which on antioxidants molecules plant-derivatives can act synergistically in antimicrobial combinations, a valuable aid as bacterial resistance modifying compounds.

Graphical Abstract

Keywords:

• Cinnamomum verum
• antibacterial
• antioxidant
• herbal drugs
• chromatographic profile
• modulatory activity

Acknowledgments

The authors would like to thank the CNPq for research grant and financial support (405297/2018-1 and 307110/2018-4), FACEPE for financial support (APQ-0493-4.03/14). They are also grateful to the CAPES.

Disclosure statement

No potential conflict of interest was reported by the authors.