Six new triterpenoids from the root of Potentilla reptans and their cardioprotective effects in silico

Abstract

Tormentic acid ester glucosides derivatives (1, 2 and 4), 3-oxoursane ester glycoside (3) and 11-methoxy-ursane ester glycosides (5, 6) as six new triterpenoids, along with catechin were isolated from the ethyl acetate fraction of Potentilla reptans root (Et) methanolic extract. The structures of the compounds were elucidated by 1D, 2D NMR, IR and MS spectroscopy. Additionally, isolated triterpenoid compounds (1–6) and catechin were evaluated for their cardioprotective effects via glycogen synthase kinase 3β (GSK-3β) and glucocorticoid regulated kinase-1 (SGK1) protein kinase inhibition by Molecular Docking. Compound 1 and catechin (compound 7) exhibited significant inhibitory effects against GSK-3β and SGK1 protein kinases with a binding energy value −9.1 and −8.8 kcal/mol, respectively. Hence, Et can be a suitable natural candidate to protect cardiomyocytes injury.

Graphical abstract

Keywords:

• Potentilla reptans root
• triterpene
• cardioprotection
• GSK-3β
• SGK1

Disclosure statement

The authors declare that they have no conflict of interest.

Authorship contributions

Protocol Design and modification: Narguess Yassa; Preparation of plant extract and phytochemical tests: Ayesheh Enayati, Aghigh Omraninava; Isolation, purification and identification of compounds: Ayesheh Enayati, Narguess Yassa; NMR spectroscopy: Mostafa Alilou, Hermann Stuppner; Molecular docking: Hasan Mirzaei; Manuscript draft: Ayesheh Enayati, Aref Salehi; Supervision: Narguess Yassa; Consultant: Aref Salehi, Vahid Khori.

Additional information

Funding

This article is derived from the author’s PhD thesis and financially supported by Golestan University of Medical Sciences, Gorgan, Iran (IR.GOUMS.REC.1394.149, 94.61.138) and Tehran University of Medical Sciences, Tehran, Iran (IR.TUMS.REC.1394.918, 94-02-33-29400).