Abstract
Phytochemical investigation of Sophora secundiflora alkaloid fraction led to isolation of one new quinolizidine alkaloid (1) 13-methoxyanagyrine together with six known ones (2–7). The insecticidal activity of 70% methanol extract of leaves of S. secundiflora, S. tomentosa and the isolated alkaloids were assessed against 3rd instar larvae of Culex pipiens (Diptera: Culicidae) using different concentrations and mortality rate was recorded. Sophora tomentosa extract showed highest mortality rate with median lethal concentration LC50 3.11 ppm after 24 h and 0.66 ppm after 48 h and anagyrine (6) exhibited remarkably insecticidal activity with LC50 value of 3.42 ppm after 24 h of exposure. Additionally, cytotoxic activity of alkaloid fraction of S. secundiflora, S. tomentosa and isolated alkaloids was also studied using crystal violet assay against MCF-7 and HEPG-2 cell lines. Anagyrine (6) exhibited IC50 values of 27.3 ± 0.7 and 30.2 ± 0.9 µg/mL against MCF-7 and HEPG-2 cancer cells, respectively.
Graphical Abstract
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Disclosure statement
No potential conflict of interest was reported by the authors.
Author contributions
(S.H.A): conceptualization, data curation, writing the original draft. (A.M.E.): validation, investigation, supervision, manuscript reviewing & editing. (A.E.A.): supervision, manuscript reviewing & editing. (S.M.A.): biological investigation, writing the original draft. (O.A.E.): visualization, supervision, manuscript reviewing & editing. (M.A.E.): visualization, supervision, manuscript reviewing & editing. (A.B.S.): visualization, supervision, manuscript reviewing & editing, project administration.