Anti-inflammatory and antioxidant activities of chemical constituents from the flower buds of Buddleja officinalis

Abstract

Five new glycosides including mimenghuasu A and B (1-2), isolinarin (3), cyclocitralosides A and B (4–5), along with forty-seven known compounds were isolated from the flower buds of Buddleja officinalis. These structures were elucidated by extensive spectroscopic analysis (UV, IR, 1 D, 2 D NMR, and MS spectra). The anti-inflammatory activities of the isolated compounds were determined by enzyme-linked immunosorbent assay (ELISA) on the expression of TNF-α (LPS-activated RAW264.7 cells) and MTT experiment on LPS-induced HUVECs proliferation effects. Good suppressive effects on the expression of TNF-α were shown by 4 and 5 with IC₅₀ values of 19.35 and 22.10 μM, respectively, compared to positive control indomethacin (IC₅₀ 16.40 μM). In addition to this, some isolated compounds exhibited excellent antioxidant activities including compounds 16, 18, 29, 39, and 47 (IC₅₀ μM: 82.59, 72.94, 33.65, 46.67, and 20.81, respectively) with almost the same or stronger potency with reference to vitamin C as positive control (IC₅₀ 81.83 μM).

Graphical Abstract

Keywords:

• Buddleja officinalis
• Scrophulariaceae
• mimenghua
• chemical constituents
• glycosides
• anti-inflammatory
• anti-oxidation

Acknowledgments

This study was supported by the National Natural Science Foundation of China (81574031), the State Key Subject of TCM diagnostics in Hunan University of Chinese Medicine (2015ZYZD06), Scientific research fund of administration of traditional Chinese medicine of Hunan Province (201857), and Changsha Science and Technology Bureau (kq1901095).

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This study was supported by the National Natural Science Foundation of China (81574031), the State Key Subject of TCM diagnostics in Hunan University of Chinese Medicine (2015ZYZD06), Scientific research fund of administration of traditional Chinese medicine of Hunan Province (201857), and Changsha Science and Technology Bureau (kq1901095).