In vitro anti-PRV activity of dihydromyricetin from Ampelopsis grossedentata

Abstract

Pseudorabies (PR) is an acute infectious disease caused by pseudorabies virus (PRV). There are no available drugs due to the emergence of variant of PRV. Dihydromyricetin (DMY) is a flavonoid extracted from Ampelopsis grossedentata (A. grossedentata), which has a variety of pharmacological activities. In this study, we aim to investigate the in vitro anti-PRV activity of DMY extracted from A. grossedentata. MTT assay was used to detect the cytotoxicity and antiviral activity of DMY. The results detected by flow cytometry and qRT-PCR showed that DMY played anti-PRV role mainly by interfering with the process of virus invasion into host cell and inhibiting the occurrence of pyroptosis in vitro. This suggested that anti-pyroptosis may be an important antiviral mechanism for DMY which is expected to be a potential anti-PRV drug.

Graphical Abstract

Keywords:

• Pseudorabies virus
• dihydromyricetin
• pyroptosis

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by Guizhou Science and Technology Planing Project (grant no. [2019]1456).