Antimelanogenic activity of patuletin from Inula japonica flowers in B16F10 melanoma cells and zebrafish embryos

Abstract

During the search for natural melanogenesis inhibitors, patuletin, a flavonoid, was isolated from Inula japonica flowers. We investigated the antimelanogenic effects of patuletin on B16F10 melanoma cells and zebrafish embryos. Patuletin dose-dependently reduced melanocyte-stimulating hormone-induced melanogenesis and L-DOPA oxidation in B16F10 cells. Western blot analysis showed that patuletin reduced cellular tyrosinase expression in a dose-dependent manner. Patuletin treatment significantly decreased melanin pigmentation in the embryo compared to the untreated controls

Graphical Abstract

Keywords:

• Inula japonica
• patuletin
• melanogenesis
• B16F10 melanoma cells
• zebrafish embryos

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This research was supported by a grant (KSN2013330) from the Korea Institute of Oriental Medicine.