Phytochemical investigation and biological activity of Doronicum pardalianches L. roots against Alzheimer’s disease

Abstract

The methanol extract of Doronicum pardalianches L. was fractionated using n-hexane, chloroform, and ethyl acetate to evaluate their cholinesterase (ChE) inhibitory activity via modified Ellman’s method. It was perceived that only the ethyl acetate fraction was active toward acetylcholinesterase (AChE) with IC₅₀ value of 172.21 µg/mL. Also, all fractions showed no butyrylcholinesterase (BChE) inhibitory activity. The ethyl acetate fraction was also investigated for its neuroprotectivity and metal chelating ability (Zn²⁺, Fe²⁺, and Cu²⁺) which demonstrated desired activity. Phytochemical analysis of the ethyl acetate fraction led to isolation and identification of formononetin 7-O-β-D-glucopyranoside which has not been previously reported for this plant.

Graphical Abstract

Keywords:

• AChE
• BChE
• Doronicum pardalianches
• metal chelating
• neuroprotectivity
• phytochemistry

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by grants from the Research Council of Tehran University of Medical Sciences with project No. 99-2-157-49769.