Dismantling Parkinson’s disease with herbs: MAO-B inhibitory activity and quantification of chemical constituents using HPLC-MS/MS of Egyptian local market plants

Abstract

Withania somnifera, Angelica sinensis, Glycyrrhiza glabra, and Simmondsia chinensis were acquired from the Egyptian market, profiled for their chemical constituents, screened for the in-vitro MAO-B inhibitory activity and evaluated for the total phenolic content. Thirty compounds were characterized in the selected herbs using HPLC-MS/MS. In-vitro MAO-B inhibitory activity and total phenolic content of the acquired herbs were compared with those of a prepared herbal formula consisting of a mixture of equal amounts of the four mentioned herbs. The most potent MAO-B inhibitory activity was exerted by the methanol extract of the prepared formula (IC₅₀ of 712.19 ± 13.90 ng/mL) compared to selegiline (IC₅₀ of 581.69 ± 11.35 ng/mL). The highest value of the total phenolic content was shown by Angelica sinensis methanolic extract (76.15 ± 0.1 mg/g) followed by Glycyrrhiza glabra methanolic extract (65.74 ± 0.1 mg/g), then the mixture’s methanolic extract of the four herbs (37.04 ± 0.1 mg/g).

Graphical Abstract

Keywords:

• HPLC-MS/MS
• Ashwagandha
• Angelica
• licorice
• jojoba
• MAO-B
• Parkinson’s disease

Disclosure statement

The authors have no conflict of interest to declare.

Funding

The author(s) reported there is no funding associated with the work featured in this article.