Comparative study of four Yucca species by 2D-NMR and LC-MS

Abstract

The present study was conducted to compare leaf extracts of Y. gloriosa, Y. treculeana, Y. filamentosa and Y. aloifolia. Plant extracts were obtained by sequential extraction with 50% and 100% methanol using the pressurized liquid extraction method. General comparative analysis of Yucca extracts was performed by 2 D-NMR. The presence of various biologically active components in the extracts was shown, among which steroid saponins predominate. The use of LC-MS and LC-HRMS methods with electrospray ionization made it possible to conduct a comparative analysis of Yucca steroidal saponins. The 33 steroidal saponins of various structures were found. The presence of three characteristic saponins in all Yucca species was shown: yuccaloeside C, yuccaloeside E and yuccaloeside B. The results obtained can be used to control the quality of plant materials and products.

Graphical abstract

Keywords:

• Yucca
• steroidal saponins
• comparative study
• 2D NMR
• LC-MS
• LC-HRMS

Disclosure statement

No potential conflict of interest was reported by the authors.

Data availability statement

The data presented in this study are available in the article and Supplementary Materials (SM).

Additional information

Funding

This work was financially supported by the state assignment of Ministry of Health of the Russian Federation (№ 056-00017-21-00) and by the Ministry of Science and Higher Education of the Russian Federation (FSRU-2021-0009). Instrumentation of the Core Facility Center «Arktika» of Northern (Arctic) Federal University was used in this work.