Quantitative analysis of tiliroside and other flavonoid glycosides in Hippophae salicifolia D. Don leaves by HPLC-PDA

Abstract

A new HPLC-PDA method was developed and validated for simultaneous determination of five phenolic compounds (trans-and cis- isomers of tiliroside, quercetin-3-O-β-D-glucoside, ellagic acid, kaempferol-3-O-β-D-glucoside and isorhamnetin-3-O-glucoside) in the leaves of Hippophae salicifolia D. Don. Of the five compounds, three (tiliroside, quercetin-3-O-β-D-glucoside and ellagic acid) were isolated and characterised by spectroscopy techniques. The developed HPLC method provided a selective, sensitive and rapid analysis with good linearity (r²> 0.999), accuracy and precision. Also, the leaves of H. salicifolia were extracted by three different extraction techniques viz. reflux, microwave and ultrasound. Methanolic extracts prepared by reflux method showed the highest content of all the five compounds.

Graphical Abstract

Keywords:

• Hippophae salicifolia
• Leh berry
• phenolics
• HPLC-PDA
• tiliroside

Acknowledgements

We are thankful to the Dr. Dinesh Kumar, IHBT palampur for collecting and providing the plant material.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

The authors are grateful to Department of Biotechnology (DBT) Govt of India for financial assistance (BT/PR9116/NDB/39/390/2013) and providing research fellowship to Ms. Soni Ranjana.