Aspidiatas C and D, two new spirostanol saponins from Aspidistra triradiata and their cytotoxic activities

Abstract

Aspidiatas C and D (1 and 2), two new spirostanol saponins, were isolated along with two known compounds, (25 R*)-spirost-5-en-3β-yl α-L-rhamnopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→4)-[α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranoside (3), (25 R*)-spirost-5-en-3β-yl α-L-rhamnopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→4)-β-D-glucopyranoside (4) from the whole plant of Aspidistra triradiata collected in Vietnam. The chemical structures were determined by HRESIMS, 1D- and 2D-NMR analysis, and comparison with published data. Compound 3 exhibited potent cytotoxicity against MCF7, HepG2, SK-LU-1, and HT-29 human cancer cell lines with IC₅₀ values ranging from 0.19 to 0.65 µM. Compounds 1, 2, and 4 displayed moderate cytotoxic effects with IC₅₀ values ranging from 12.32 to 82.27 µM. Compounds 1–4 were isolated from the genus Aspidistra for the first time.

GRAPHICAL ABSTRACT

Keywords:

• Aspidiata C
• aspidiata D
• Aspidistra triradiata
• cytotoxic activities

Acknowledgments

We are grateful to Mr. Luong Vu Dang (Institute of Chemistry, VAST, Hanoi, Vietnam) for recording the NMR spectra.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was financially supported by Hue University under Grant (ID No. DHH2022-04-160). Le Thi Bich Hien was funded by the Master, PhD Scholarship Programme of Vingroup Innovation Foundation (VINIF), code VINIF.2022.TS039.