Enhanced uptake of Cd by biofilm forming Cd resistant plant growth promoting bacteria bioaugmented to the rhizosphere of Vetiveria zizanioides

Abstract

Heavy metals are the major cause of pollution and cadmium is one among the highly toxic metals discharged into the environment from various industries. The current study was focused on the bioremoval of cadmium by phyto and rhizoremediation approach using Vetiveria zizanioides. The bacterial strains were isolated from wetland paddy rhizosphere soil and the isolate VITJAN13 was found to be a biofilm forming Cd resistant plant growth promoting rhizobacteria (PGPR). The 16S rRNA gene sequencing revealed VITJAN13 to be the closest neighbor of Aeromonas sp. and was submitted to Genbank with the accession number KX770741. Further, pot culture studies indicated that the treatments bioaugmented with VITJAN13 increased the root length and shoot height by 21.4 and 17.36%, respectively as compared to the non-augmented plants. Hence, bioaugmentation of Aeromonas sp. in the rhizosphere of Vetiveria zizanioides enhanced the uptake of cadmium by 67.7% in the soil treated with 15 mg/kg of Cd to that of the phytoremediation setup.

Keywords:

• Cadmium
• phytoremediation
• rhizoremediation

Acknowledgment

The authors are grateful to VIT and its management for providing lab facilities for conducting our research. The authors are thankful to the TBI – VIT for providing AAS facility and nursery (VIT) for the space provided towards pot culture study.