The photosynthetic physiological response and purification effect of Salix babylonica to 2, 4-dinitrophenol wastewater

Abstract

Phytoremediation technology based on living green plants would clean up water pollution. Through hydroponic experiment, the effects of different concentration of 2, 4-dinitrophenol (2, 4-DNP) on the photosynthetic and chlorophyll fluorescence parameters of Salix babylonica, and the absorption and purification effect of S. babylonica on 2, 4-DNP were measured to explore the tolerance of S. babylonica to 2, 4-DNP and the feasibility to purify dinitrophenol waste water by it. The biomass, actual photochemical efficiency (PSII), net photosynthetic rate (Pn), photochemical quenching coefficient (qP), stomatal conductance (Gs), transpiration rate (Tr), maximum photochemical efficiency (Fv/Fm) and chlorophyll content of the S. babylonica showed downward trend with the increasing exposure concentrations of 2,4-DNP, but the intercellular CO₂ concentration (Ci) appeared upward trend. Non-photochemical quenching coefficient (NPQ) increased at 5 mg L^–1, then declined with the increase concentrations of 2, 4-DNP. In addition, the percent removal of 2, 4-DNP in 20 mg L^–1 waste water was 91.4%. In conclusion, 2, 4-DNP significantly inhibits Pn of S. babylonica and the reduction of Pn was caused by decreasing Gs, carboxylation efficiency and chlorophyll content. When the concentration of 2, 4-DNP is not more than 20 mg L^–1, S. babylonica can remove 2, 4-DNP efficiently.

Keywords:

• 2, 4-DNP stress
• photosynthesis
• phytoremediation
• S. babylonica

Additional information

Funding

The present research was financially supported by Science and Technology Fund Project of Shandong Provincial Department of Agriculture and Rural Affairs.