ABSTRACT
The current study aims to determine the inhibition activity gelatin against dipeptidyl aminopeptidase 4 (DP-4). Two commercial gelatins, i.e., bovine and fish skin gelatin and one extracted (in our laboratory) gelatin, i.e., fish bone gelatin were selected for analysis. Each gelatin have same protein pattern (75–245 kDa) on sodium dodecyl sulfate polyacrylamide gel electrophoresis with mean of protein concentration of 1.72 mg/mL. The inhibition activity was measured on the capacity to inhibit DP-4 by using Gly-Pro-p-nitroanilide as their substrate. The sitagliptin was used as standard comparison. Based on the percent inhibition, gelatin has been shown to be the prospective DP-4 inhibitor.
Acknowledgments
This research was supported by Grant of Directorate General of Research and Development in Penelitian Kerjasama Antar Perguruan Tinggi (PKPT) scheme, Ministry Research Technology and Higher Education of Indonesia
Correction Statement
This article has been republished with minor changes. These changes do not impact the academic content of the article.