Morphometric evaluation of Japanese quail embryos and their extraembryonic vascular networks exposed to low-frequency magnetic field with two different intensities

ABSTRACT

Animals developed or in an embryonic stage, are constantly subjected to magnetic pollution generated by electrical and electronic devices. Several researches have used the bird embryo as an experimental model to evaluate the action of magnetic field (MF) and electromagnetic field (EMF). This study proposed to perform a morphometric evaluation in the embryos and in the blood vascular network of the yolk sac membranes (YSM) of Japanese quail (Coturnix japonica) exposed to the 60 Hz MF with two different intensities (0.16 and 0.65 mT). A total of 30 eggs were used, 10 eggs were used for each assay. Each assay formed a group (control group, group submitted to the MF of 0.16 mT and 0.65 mT). The images of the skeletonized vascular network of YSM were evaluated by two methods of fractal dimension: box-counting dimension (D_bc) and information dimension (D_inf). The embryos were evaluated by body mass, percentage cephalic length and body area. The fractal dimensions revealed no difference among groups. There were no significant differences in relation to embryonic body mass among groups. However, the embryos exposed to 0.65 mT MF presented a smaller embryonic body development (body area and percentage cephalic length). In conclusion, 0.16 mT and 0.65 mT magnetic fields were not able to generate significant effects on vasculogenesis and angiogenesis. However, the embryos exposed to 6 h of magnetic field with 0.65 mT intensity and 60 Hz frequency showed a decrease in embryonic body development.

KEYWORDS:

• Vascular network
• fractal dimension
• magnetic field
• yolk sac membrane
• quail embryo

Declaration of interest

The authors report no conflicts of interest.

Additional information

Funding

This work was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico [CNPQ n.6/2019]; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior [PNPD/CAPES]; Fundação de Amparo a Ciência e Tecnologia do Estado de Pernambuco [BCT-0183-2.09/15].