Abstract
Ulcerative Colitis (UC) is a disease that negatively affects quality of life and is associated with sustained oxidative stress, inflammation and intestinal permeability. Vitamin D and Curcumin; It has pharmacological properties beneficial to health, including antioxidant and anti-inflammatory properties. Our study investigates the role of Vitamin D and Curcumin in acetic acid-induced acute colitis model. To investigate the effect of Vitamin D and Curcumin, Wistar-albino rats were given 0.4 mcg/kg Vitamin D (Post-Vit D, Pre-Vit D) and 200 mg/kg Curcumin (Post-Cur, Pre-Cur) for 7 days and acetic acid was injected into all rats except the control group. Our results; colon tissue TNF-α, IL-1β, IL-6, IFN-γ and MPO levels were found significantly higher and Occludin levels were found significantly lower in the colitis group compared to the control group (p < 0.05). TNF-α and IFN-γ levels decreased and Occludin levels increased in colon tissue of Post-Vit D group compared to colitis group (p < 0.05). IL-1β, IL-6 and IFN-γ levels were decreased in colon tissue of Post-Cur and Pre-Cur groups (p < 0.05). MPO levels in colon tissue decreased in all treatment groups (p < 0.05). Vitamin D and Curcumin treatment significantly reduced inflammation and restored the normal histoarchitecture of the colon. From the present study findings, we can conclude that Vitamin D and Curcumin protect the colon from acetic acid toxicity with their antioxidant and anti-inflammatory potential.
Brief synopsis: In this study; distal colon, distal ileum, jejunum and serum physiopathology in colitis induced by acetic acid and intestinal permeability were investigated. The roles of vitamin D and curcumin in this process were evaluated.
Author contributions
Idea/Concept: ASE; Design: ASE, OC; Data Collection/Processing: ASE, OC, FS, EG; Analysis/Interpretation: FS, ASE; Literature Review: ASE; Drafting/Writing: ASE; Critical Review: RO, GC.
Acknowledgments
I would like to thank Aydın Adnan Menderes University Scientific Research Projects Unit for their support (Grant Number: TPF-20014). I would like to thank Assoc. Prof. Rahime Aslankoc for her critical review support, Res. Assist. Burcin İrem Abas for her support in biochemical analyzes, Dr. Cenk Orak for his support on the experimental idea/concept, Erdag Gönen for his support in supplying curcumin. I would like to thank Res. Assist. Bayram Talay Deniz, Res. Assist. Oguzhan Kavrik and Res. Assist. Ozge Beyazcicek for their support.
Disclosure statement
No potential conflict of interest was reported by the author(s).
Data availability statement
The data used to support the findings of this study belong to the Aydın Adnan Menderes University, Faculty of Medicine, Department of Physiology and are available upon reasonable request to the corresponding author at [email protected].