Immunohistochemical Panels to Evaluate Important Immunophenotypes of Human Mesonephros

Abstract

Background. The immunophenotypes and potential excretory function of human mesonephros are not well studied. Methods. Five mesonephros specimens of human embryos from the 6^th to 10^th weeks of gestation were stained with immunohistochemical markers. Results. PAX8 was universally expressed in all renal tubules, while α-methyacyl-CoA racemase (AMACAR) was positive in proximal tubules and GATA3 was positive in distal tubular mesonephric structures. At the 8th weeks of gestation, the mesonephric glomeruli were characterized by opened glomerular capillary loops with Periodic Acid Schiff (PAS)-positive glomerular basement membranes and GATA3-positive mesangial-like cells. By the 8^th week, proximal tubules showed PAS-positive brush borders, indicating reabsorption capacity, and the proximal tubules also demonstrated positivity with kidney injury molecule-1 (KIM-1), representing tubular response to injury. Conclusion. Our overall findings show detailed phenotypes of the glomerular and tubular structures of the mesonephros and indicate that at the 8^th week of gestation, the mesonephros may carry out temporary excretory function before metanephros becomes fully functional.

Keywords:

• Mesonephros
• metanephros
• kidney injury molecule-1
• brush borders
• GATA-3
• differentiation
• maturation

Acknowledgments

Authors thank Beaumont Histology and Immunohistochemistry labs for their excellent technical support.

Disclosure statement

The authors declare no conflicts of interests

Funding

The author(s) reported there is no funding associated with the work featured in this article.