Repeatability and reproducibility of anterior lens zonule length measurement using ArcScan insight 100 very high-frequency ultrasound

ABSTRACT

Objectives

This study aimed to evaluate the intra-examiner repeatability and inter-examiner reproducibility in lens zonular length measurements using very high-frequency digital ultrasound (Insight 100).

Methods

Two examiners performed ultrasound imaging independently in each subject. The length of temporal and nasal zonules were then measured with a built-in software. Coefficient of variations (CVs) of the three repeated measurements were used to determine intra-examiner variances. Inter-examiner reproducibility was evaluated using intraclass correlation coefficients (ICCs) and the Bland-Altman method.

Results

40 eyes of 40 subjects (14male and 26female; mean age 23.9 ± 2.4 years) were included in the study. The CVs for intra-examiner measurement were 2.74% temporally and 4.32% nasally for Examiner 1, and were 1.96% temporally and 1.75% nasally for Examiner 2. For inter-examiner reproducibility, all ICCs were above 0.9. However, there were significant differences between the two examiners in temporal zonular length measurements (p = 0.001), and the differences mainly came from measuring the zonular length manually (p = 0.001) rather than recording images (p = 0.480). No significant differences were found between two measurements by the same examiner after one month (all p > 0.05, all ICCs > 0.8).

Conclusion

The Insight 100 device can be used to measure the length of anterior lens zonule with relatively good repeatability and reproducibility.

Clinical trial registration

www.clinicaltrials.gov identifier is NCT05657951

KEYWORDS:

• Insight-100
• lens zonule
• repeatability
• reproducibility
• very high-frequency ultrasound

Acknowledgments

We would like to thank Editage (www.editage.jp) for English language editing services.

Declaration of interest

The authors have no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties.

Declaration of interest

Peer reviewers on this manuscript have no relevant financial relationships or otherwise to disclose.

Author contribution

Design of the study (ZH Zhu, YE Zhao); conduct of the study, data collection (ZH Zhu, H Zou, HZ Li, XE Wu, YY Wang); data analysis (ZH Zhu); drafting the manuscript (ZH Zhu); revising the manuscript critically for important intellectual content (YE Zhao).

Additional information

Funding

This paper was funded by National Science Foundation of Zhejiang Province (LQ20H120002) and Zhejiang Medical and Health Science and Technology Plan (2023KY913).