Nanopolystyrene beads affect motility and reproductive success of oyster spermatozoa (Crassostrea gigas)

Abstract

Oysters are keystone species that use external fertilization as a sexual mode. The gametes are planktonic and face a wide range of stressors, including plastic litter. Nanoplastics are of increasing concern because their size allows pronounced interactions with biological membranes, making them a potential hazard to marine life. In the present study, oyster spermatozoa were exposed for 1 h to various doses (from 0.1 to 25 µg mL⁻¹) of 50-nm polystyrene beads with amine (50-NH₂ beads) or carboxyl (50-COOH beads) functions. Microscopy revealed adhesion of particles to the spermatozoa membranes, but no translocation of either particle type into cells. Nevertheless, the 50-NH₂ beads at 10 µg mL⁻¹ induced a high spermiotoxicity, characterized by a decrease in the percentage of motile spermatozoa (−79%) and in the velocity (−62%) compared to control spermatozoa, with an overall drop in embryogenesis success (−59%). This major reproduction failure could be linked to a homeostasis disruption in exposed spermatozoa. The 50-COOH beads hampered spermatozoa motility only when administered at 25 µg mL⁻¹ and caused a decrease in the percentage of motile spermatozoa (−66%) and in the velocity (−38%), but did not affect embryogenesis success. Microscopy analyses indicated these effects were probably due to physical blockages by microscale aggregates formed by the 50-COOH beads in seawater. This toxicological study emphasizes that oyster spermatozoa are a useful and sensitive model for (i) deciphering the fine interactions underpinning nanoplastic toxicity and (ii) evaluating adverse effects of plastic nanoparticles on marine biota while waiting for their concentration to be known in the environment.

Keywords:

• Nanoplastics
• oyster
• spermatozoa
• motility
• reproductive success

Acknowledgments

This study was supported by the ANR-Nanoplastics project (ANR-15-CE34-0006). K. Tallec has a French doctoral grant from the ‘Région Bretagne’ (50%) and Ifremer (50%). The authors thank E. Fleury, M. Suquet, J. L. Seugnet, and the staff of the experimental station of Argenton for their technical assistance. We thank A. Donval for TEM preparation, V. Foulon, N. Gayet, and the Imaging Platform of UBO (Plateforme d’imagerie et de Mesures en Microscopie [PIMM]) for the help with microscopy analyses. We thank H. McCombie for her help in editing the English.

Disclosure statement

No potential conflict of interest was reported by the author(s).