Aflatoxin B₁ in peanut oil from Western Guangdong, China, during 2016–2017

ABSTRACT

Aflatoxin B₁ (AFB₁) occurrence in peanut oil samples randomly collected from family workshops in western Guangdong during 2016–2017 (n = 427) was surveyed. AFB₁ content was screened by enzyme-linked immunosorbent assay (ELISA) protocol and analytically confirmed with an UPLC-MS/MS method. The limit of detection of the ELISA method was 1.08 μg kg⁻¹. The recovery values ranged from 84.4 to 92.6% with relative standard deviations of 2.2 to 4.8%. AFB₁ was quantified in 47 samples (22.5%) with a range of 15.4–49.9 μg kg⁻¹ in 2016 and in 33 samples (15.1%) with 8.8–22.2 μg kg⁻¹ in 2017, respectively. The AFB₁ contamination in peanut oil was season-depended in western Guangdong with the worst case in spring (24.2–37.9%). Overall, a significant reduction of AFB₁ occurrence was observed in western Guangdong after 2016. It is advisable to control AFB₁ in bulk and self-pressed oil from family workshops and regulate it mandatorily if necessary.

KEYWORDS:

• Aflatoxin B₁
• peanut oil
• ELISA
• UPLC-MS/MS
• family workshops

Acknowledgments

We are grateful to Mrs. Lili HE and Mr. Qing AI (R-Biopharm Analysis Systems Trading (Beijing) Co., Ltd.) for the assistance during the validation of analytical methods.

Disclosure statement

No potential conflict of interest was reported by the authors.

Supplementary material

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Additional information

Funding

This work was supported by the National Project for Agro-product Quality & Safety Risk Assessment (GJFP 2018011) and Hainan Provincial Natural Science Foundation of China (318QN260).