Preparative isolation and purification of B-type fumonisins by using macroporous resin column and high-speed countercurrent chromatography

ABSTRACT

B-type fumonisins (FBs) are water-soluble mycotoxins produced by Fusarium species, which are mainly found in maize products and threaten food safety. Toxicological studies and quantitative determinations of fumonisins require large amounts of pure toxins, and their high prices limit progress in FBs research. In this study, we used a macroporous resin column combined with high-speed countercurrent chromatography to separate large quantities of FBs. A fermented rice culture was extracted with 75% methanol. The dynamic adsorption capacity of FBs on XAD-2 resin was 27.5 mg/g resin at 25°C, pH 4.0, and then the FBs were desorbed with 60% methanol. The crude FBs were further purified using a biphasic system consisting of n-heptane/n-butanol/methanol/water (2:4:1:4, v/v/v/v). The method yielded 1.55 g of FB1 and 0.55 g of FB3 with purities of 96.8% and 95.6%, respectively, from 1 kg of rice culture, and the final overall yield of FBs was 74.8%.

GRAPHICAL ABSTRACT

KEYWORDS:

• Fumonisin
• macroporous resin
• high-speed countercurrent chromatography

Disclosure statement

No potential conflict of interest was reported by the authors.

Supplementary material

Supplemental data for this article can be accessed on the publisher’s website.

Additional information

Funding

This work was financially supported by the National Natural Science Foundation of China (31872914, U1604234), Jiangsu Agriculture Science and Technology Innovation Fund (CX(17)1003) and International Science & Technology Cooperation Program of China (2016YFE0112900), National Key R&D Program of China (2018YFD0200500).