Epidemiology and antibiotic susceptibility in anaerobic bacteraemia: a 15-year retrospective study in South-Eastern Hungary

Abstract

Introduction

Bloodstream infections are considered as important factors of morbidity and represent a remarkable number of death, especially in developed countries.

Methods

In a 15-year retrospective study in the South-Eastern region of Hungary, the microbiological results of anaerobic blood culture (BC) bottles were analysed. The incubation of BCs was performed in the automated incubation system, and the isolates were cultured in an anaerobic chamber. The identification of the isolates was based on their biochemical profile (2004–2012) and mass spectrometry analyses (2013–2018). Antibiotic susceptibility testing was performed with minimal inhibitory concentration gradient diffusion method.

Results

The majority of the samples were submitted by the Department of Internal Medicine (37.7%), Intensive Care Unit (31.6%) and the Emergency Department (16.4%), and the number of strict anaerobic isolates per year showed an increasing tendency throughout the study. More than half of the isolates were Cutibacterium acnes (54.4%), but this bacterium was considered as a contaminant. The most frequent species upon Cutibacterium acnes were Bacteroides fragilis (9.2%), Clostridium perfringens (6.4%), other Clostridium species (4.8%), other Cutibacterium species (3.5%) and other Bacteroides species (2.5%). Penicillin, amoxicillin/clavulanic acid, imipenem, clindamycin, metronidazole and tigecyclin were very effective against Bacteroides fragilis, Clostridium perfringens, Fusobacterium nucleatum, Gemella morbillorum, Parvimonas micra and Eggerthella lenta strains.

Conclusion

We performed a 15-year period, retrospective investigation in South-Eastern Hungary with the participation of more than 1000 patients. The results of antibiotic susceptibility tests are in line with previous reports in the literature, and the results from the different geographical regions of the world suggest similar resistance mechanisms.

Keywords:

• Anaerobic bacteraemia
• blood culture
• antibiotic susceptibility

Ethical approval

The survey was conducted in accordance with the Declaration of Helsinki and national and institutional ethical standards. Ethical approval for the study protocol was obtained from the Human Institutional and Regional Biomedical Research Ethics Committee, University of Szeged (registration number: 4654).

Disclosure statement

No potential conflict of interest was reported by the author(s).