DNA barcoding of shrimps from a mangrove biodiversity hotspot

Abstract

A total of 74 shrimp specimens were sequenced at a 584 bp segment of the cytochrome oxidase subunit I (COI) gene to examine patterns of DNA barcode variation in a mangrove biodiversity hotspot. The Maximum Likelihood tree, barcode gap analysis, Automatic Barcode Gap Discovery analysis and sequence comparisons with data available from Barcode of Life Data System and GenBank recovered 18 taxa of which 15 were identified to species level, 2 at genus level and a single taxon at order level. Two deep mitochondrial DNA lineage divergences were found in the giant tiger prawn, Penaeus monodon. It is suggested that one of the lineages is a consequence of an introduction from aquaculture activity. These results have provided a reliable barcode library for cataloguing shrimps in this area.

Keywords:

• Shrimp
• Kedah
• Strait of Malacca
• DNA barcoding
• cytochrome oxidase subunit I (COI)

Acknowledgements

The authors are grateful to Mohamad Fhaizul MB, Suhaimi S, and Mohd Muhaznizee CM for helping with the collection of specimens.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

We would like to acknowledge the Ministry of Higher Education, Universiti Sains Malaysia [203/PBIOLOGI/6711455 and 1001/PBIOLOGI/870018] and the Ministry of Water, Land and Natural Resources (KATS) [304/PBIOLOGI/650878/K130] for research funding.