Decreased S1P and Sphk2 Are Involved in Pancreatic Acinar Cell Injury

Abstract

Aim: The roles of S1P in acute pancreatitis (AP) or non-AP patients with pancreatic acinar cell injury (PACI) are not well understood. Materials & methods: Serum S1P, in 40 healthy individuals and 99 patients with PACI, was retrospectively analyzed. Additionally, we detected and analyzed S1P in AP mice and the AR42J acinar cell line. Results: Serum S1P was significantly decreased in PACI patients, compared with that of healthy controls. Patients with gall stones, normal serum calcium or normal blood lipids showed relative higher levels of serum S1P. Interestingly, in patients with gall or liver disease, serum S1P was positively associated with γ-GT and ALT. Additionally, S1P and SPHK2 were decreased in AP mice and AR42J cells, relative to the levels of corresponding controls. Conclusion: Serum S1P is decreased in PACI, which may be partly due to downregulation of pancreatic SPHK2.

Keywords:

• γ-GT
• ALT
• AP mice
• AR42J
• gall stones
• hyperlipemia
• PACI
• S1P
• serum calcium
• SPHK2

Financial & competing interests disclosure

This study was supported in part by the National Natural Science Foundation of China (grant numbers 81572738 and 81800576), the Natural Science Foundation of Guangxi (grant number 2015GXNSFEA139003), the Lijiang Scholar Award in Guilin, the High Level of Innovation Team and Outstanding Scholars Program in Colleges and Universities in Guangxi, the Hundred Talents Program ‘the Introduction of Overseas High-Level Talents in Colleges and Universities in Guangxi’, and the recruitment program for the Affiliated Hospital of Guilin Medical University. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

All animal care and experimental procedures were approved by the Ethical Committee on Animal Experiments at Guilin Medical University. This project was conducted in the Clinical Research Center under the supervision and assessment by the Ethics committee, The Affiliated Hospital of Guilin Medical University.

Additional information

Funding

This study was supported in part by the National Natural Science Foundation of China (grant numbers 81572738 and 81800576), the Natural Science Foundation of Guangxi (grant number 2015GXNSFEA139003), the Lijiang Scholar Award in Guilin, the High Level of Innovation Team and Outstanding Scholars Program in Colleges and Universities in Guangxi, the Hundred Talents Program ‘the Introduction of Overseas High-Level Talents in Colleges and Universities in Guangxi’, and the recruitment program for the Affiliated Hospital of Guilin Medical University. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.