Circular RNA CircTTBK2 Facilitates Non-Small-Cell Lung Cancer Malignancy through the miR-873-5p/TEAD1/DERL1 Axis

Abstract

Aim: The underlying mechanisms by which circular RNAs (circRNAs) regulate non-small-cell lung cancer (NSCLC) progression remain elusive. This study investigated the role of circRNA circTTBK2 in NSCLC tumorigenesis. Materials & methods: Quantitative reverse transcriptase polymerase chain reaction analysis of circTTBK2 in NSCLC tissues and cell lines was performed. Cell proliferation, migration, invasion and tumorigenesis were confirmed in vitro and in vivo using CCK-8, EdU incorporation, Transwell assays and xenograft technique. The circTTBK2/miR-873-5p/TEAD1/DERL1 axis was verified by RNA immunoprecipitation, chromatin immunoprecipitation and luciferase reporter assays. Results: Overexpressed circTTBK2 in NSCLC tissues indicates poor prognosis of NSCLC patients. circTTBK2 harbors miR-873-5p, and miR-873-5p directly targets TEAD1. TEAD1 transcriptionally activates DERL1. Conclusion: This study revealed a novel machinery of circTTBK2/miR-873-5p/TEAD1/DERL1 for NSCLC tumorigenesis.

Keywords::

• circTTBK2
• DERL1
• miR-873-5p
• NSCLC
• TEAD1

Supplementary data

To view the supplementary data that accompany this paper please visit the journal website at: www.tandfonline.com/doi/suppl/10.2217/epi-2021-0480

Author contributions

Guarantor of integrity of the study: G-P Meng. Study concepts: J-Y Wei, G-P Meng. Study design: J-Y Wei, G-P Meng. Definition of intellectual content: J-Y Wei, Q Zhang. Literature research: Y Yao. Experimental studies: J-Y Wei, H-B He. Data acquisition: C-H Sun. Data analysis: T-T Dong. Statistical analysis: Y Yao. Manuscript preparation: J-Y Wei. Manuscript editing: J-Y Wei, G-P Meng

Financial & competing interests disclosure

The authors have no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This study was supported by General Programs of National Natural Science Foundation of China (No.81672297). This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

This study was approved by the Ethics Committee of the Second Hospital of Jilin University and followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations. In addition, for investigations involving human subjects, informed consent has been obtained from the participants involved.

Additional information

Funding

The authors have no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This study was supported by General Programs of National Natural Science Foundation of China (No.81672297). This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties. No writing assistance was utilized in the production of this manuscript.