Abstract
Aim: We aimed to determine the role of granulosa cells (GCs) circular RNA (circRNA) in ovarian aging. Methods: Nine women were recruited, including three diminished ovarian reserve young women, three advanced-aged women and three normal ovarian reserve young women. The circRNA expression profiles of GCs were characterized by CLEAR software. Key circRNA were validated by quantitative reverse transcription PCR. Results: GCs in advanced-age group females exhibited active MHC class II-related biological processes. A total of 3575 circRNAs were found in the advanced age group. Hsa-circ-0031584 appears to be one of the important molecules regulating the mitotic process of GCs. Conclusion: The expression profiles of circRNAs exhibited obvious stage specificity with age which might contribute to ovarian aging progression.
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Supplementary data
To view the supplementary data that accompany this paper please visit the journal website at: www.tandfonline.com/doi/suppl/10.2217/epi-2022-0211
Author contributions
R Huang carried out the study. P Chen analyzed and interpreted the data. W Li drafted the manuscript and collected the samples. X Liu, Y Wang and H Mai collected the samples, followed up and collected the clinical data. R Huang, P Chen and W Li coordinated the study, participated in the design and reviewed the manuscript. All authors read and approved the final manuscript.
Financial & competing interests disclosure
This research was supported and funded by the Natural Science Foundation of Guangdong Province (grant no. 2019A1515011764). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.
No writing assistance was utilized in the production of this manuscript.
Ethical conduct of research
The Ethics Committee approved the study of Sixth Affiliated Hospital of Sun Yat-Sen University (2021ZSLYEC-109). The authors state that they have obtained verbal and written informed consent from the patient/patients for the inclusion of their medical and treatment history within this case report.
Data sharing statement
The transcriptome sequencing for granulosa cells have been deposited with China National Center for Bioinformation (https://ngdc.cncb.ac.cn/) under reference no. PRJCA010954.