Dna Hypomethylation And Upregulated LINC00518 Acts As A Promoter And Biomarker In Head And Neck Squamous Cell Carcinoma

Abstract

Background: LINC00518 acts as an oncogene in several cancers, but its function in head and neck squamous cell carcinoma (HNSCC) remains unclear. Materials & methods: The expression and methylation status of LINC00518 were analyzed by reviewing public databases. The ceRNA network and the relationship with tumor immunity of LINC00518 were analyzed using online tools and in vitro studies. Results: Upregulated LINC00518 was associated with poor clinicopathological characteristics of HNSCC. Silencing LINC00518 significantly inhibited the migration of HNSCC cells. LINC00518 might positively regulate HMGA2 via the ceRNA mechanism. Additionally, LINC00518 was negatively correlated with various immune cells and immunotherapy markers. Moreover, the upregulation of LINC00518 in HNSCC may be due to DNA hypomethylation. Conclusion:LINC00518 may be a potential biomarker and therapeutic target for HNSCC.

Keywords::

• gene-based therapy
• head and neck squamous cell carcinoma (HNSCC)
• HMGA2
• hypomethylation
• lncRNAs
• tumor immunity

Supplementary data

To view the supplementary data that accompany this paper please visit the journal website at: www.tandfonline.com/doi/suppl/10.2217/epi-2023-0053

Author contributions

X Wang performed the experiments, analyzed the data and wrote the draft. J Shen and H Bo contributed to the data acquisition, analysis and interpretation. L Wang and L Deng assisted with the experiments and data. Y Luo reviewed the data and revised the manuscript. Y Cui contributed to the conception and design of the study, reviewed the data and revised the manuscript. All authors read and approved the final manuscript.

Financial & competing interests disclosure

This work was funded by the Natural Science Foundation of Hunan Province (grant no. 2020JJ5627, 2022JJ40775); the scientific research project of Hunan Health Commission (no. 202103020612). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The studies involving human participants were reviewed and approved by the Medical Ethics Committee of Changsha Medical University. The patients/participants provided their written informed consent to participate in this study.

Data sharing statement

The original contributions presented in the study are included in the article or supplementary material. Further inquiries can be directed to the corresponding author.

Additional information

Funding

This work was funded by the Natural Science Foundation of Hunan Province (grant no. 2020JJ5627, 2022JJ40775); the scientific research project of Hunan Health Commission (no. 202103020612). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.