Lineages and Sublineages of Human Papillomavirus Type 16 in Cervical Samples of Iranian Women

Abstract

Aim: This study was designed to analyze intratypic variations of human papillomavirus type 16 (HPV16) and to assess the risk of these variants for progression to cervical cancer. Materials & Methods: HPV16 variants of 58 women were determined by PCR-directed sequencing. Results: The most frequent lineage was D (67.2%) followed by A (32.8%). Lineage A was found predominantly in normal (62.5%) and cervical intraepithelial neoplasia-1 (CIN-1) (83.3%), while lineage D was the most prevalent variant in cervical cancer (100%). Conclusion: The present study revealed a distinct pattern of HPV16 variants in Iran. Based on our data, the predominant HPV16 lineage was D and there was a significant association between lineage D variants and cervical cancer.

Tweetable abstract

The intratypic variations of HPV16 were assessed in Iranian women with cervical cancer. HPV16 variants of 58 women were determined using PCR followed by directed sequencing. The most frequent lineage was D followed by A. This study revealed a distinct pattern of HPV16 variants in Iran.

Keywords::

• cervical cancer
• HPV
• HPV16
• intratype variants

Supplementary data

To view the supplementary data that accompany this paper please visit the journal website at: www.tandfonline.com/doi/suppl/10.2217/fvl-2022-0174

Acknowledgments

The authors would like to thank the directors and staff of Gynecology Oncology department and cancer institute of Imam Khomeini Hospital Complex affiliated to Tehran University of Medical Sciences for their assistance in sample collection.

Financial & competing interests disclosure

This work was supported through a grant from the Iran University of Medical Sciences and Tehran University of Medical Sciences (project code: 92-01-30-21080). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The study protocol was approved by the Ethical Committee of Tehran University of Medical Sciences. The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations. In addition, for investigations involving human subjects, informed consent has been obtained from the participants involved.

Additional information

Funding

This work was supported through a grant from the Iran University of Medical Sciences and Tehran University of Medical Sciences (project code: 92-01-30-21080). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.