Investigation of Anti-Inflammatory Effects of Oxygen Nanobubbles in A Rat Hydrochloric Acid Lung Injury Model

Abstract

Aim: To investigate the anti-inflammatory effect of oxygen nanobubbles (ONBs) in an acute lung injury rat model. Materials & methods: In a rat hydrochloric acid lung injury model, ONB fluid was administered intravenously in the ONB group (n = 6) and normal saline was administered in the control group (n = 6). 4 h later, arterial partial pressure of oxygen (PaO₂), mean arterial pressure and plasma inflammatory cytokines were measured. Results: There were no significant differences in the PaO₂, mean arterial pressure or TNF-α and IL-6 levels between the two groups. Conclusions: No anti-inflammatory effect could be confirmed at the present ONB dose in the rat model of acute lung injury.

Keywords::

• acid lung injury
• acute respiratory distress syndrome
• oxygen nanobubble
• rat model

Author contributions

K Yoshida designed the study, acquired the data and prepared the manuscript. Y Ikegami designed the study and acquired the data. S Obara, K Sato and M Murakawa contributed to coordination of the study, acquisition and analysis of data and assisted in the preparation of the manuscript. All authors have read and approved the final manuscript.

Financial & competing interests disclosure

This work was supported by JSPS KAKENHI Grant Number JP19K18332 and JP17K17061. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

Writing support was provided by the Scientific English Editing Section of Fukushima Medical University.

Ethics conduct of research

The authors state that the experimental protocol was approved by the Animal Experiment Institutional Review Board of Fukushima Medical University (approval number: 30122, Fukushima, Japan).

Data sharing statement

The data that support the findings of this study are available from the corresponding author (K Yoshida) upon reasonable request.

Acknowledgments

The authors would like to thank the Scientific English Editing Section of Fukushima Medical University for editing a draft of this manuscript.

Additional information

Funding

This work was supported by JSPS KAKENHI Grant Number JP19K18332 and JP17K17061. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.