PEGylated Single-Walled Carbon Nanotubes as Co-Adjuvants Enhance Expression of Maturation Markers in Monocyte-Derived Dendritic Cells

Abstract

Aim: This study investigated the application of phospholipid-PEGylated single-walled carbon nanotubes (PL-PEG-SWCNTs) as a safe co-adjuvant for the commercial recombinant hepatitis B virus vaccine to enhance induction of monocyte-derived dendritic cells (MDDCs) differentiation and activation in vitro as an immune response initiator cell to prompt a long-term immune response after a single dose injection. Methods: Immature MDDCs were exposed to PL-PEG-SWCNTs alone and in combination with hepatitis B vaccine. Results & conclusion: Study results confirm the enhanced expression of maturation markers in human immature MDDCs after PL-PEG-SWCNT exposure. The results suggest that PL-PEG-SWCNT is an efficient co-adjuvant for the commercial recombinant hepatitis B virus vaccine to enhance dendritic cell response stimulation in vitro.

Keywords::

• alum
• hepatitis B virus vaccine
• monocyte-derived dendritic cells
• single-walled carbon nanotubes

Supplementary data

To view the supplementary data that accompany this paper please visit the journal website at: www.tandfonline.com/doi/suppl/10.2217/nnm-2020-0339

Financial & competing interests disclosure

This study was financially supported by Pasteur Institute of Iran (PII) and Protein Technology Research Center (PTRC) with ethics number IR.PII.REC.1395.85. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

Writing support for native English editing was provided by NativeEnglishEdit.com and was funded by the authors.

Acknowledgments

The authors are grateful to M Mohammadipour from Iranian Blood Transfusion Research Center for providing of human buffy coats.

Additional information

Funding

This study was financially supported by Pasteur Institute of Iran (PII) and Protein Technology Research Center (PTRC) with ethics number IR.PII.REC.1395.85. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.