Analysis of 8q24.21 miRNA Cluster Expression and Copy Number Variation in Gastric Cancer

Abstract

Aim: To analyze gene expression and copy number of five miRNAs (miR-1204, miR-1205, miR-1206, miR-1207 and miR-1208) localized in this chromosome region in gastric cancer (GC). Materials & methods: 65 paired neoplastic and non-neoplastic specimens collected from GC patients and 20 non-neoplastic gastric tissues from cancer-free individuals were included in this study. The expression levels of the five miRNAs were accessed by real time qPCR and were correlated. Results:MiR-1207-3p, miR-1205, miR-1207-5p and miR-1208 were upregulated in approximately 50% of GC tumors in relation to those of adjacent non-neoplastic tissues. MiR-1205 expression was associated with gain of gene copies and was upregulated in adjacent non-neoplastic samples relative to external controls. Conclusion: The coexpression of the 8q24 miRNAs indicated the role of miR-1205 in the initiation of gastric cancer development.

Keywords: :

• 8q24
• CNV
• gastric cancer
• gene expression
• miRNA
• miR-1204
• miR-1205
• miR-1206
• miR-1207
• miR-1208

Supplementary data

To view the supplementary data that accompany this paper please visit the journal website at:www.tandfonline.com/doi/full/10.2217/epi-2016-0184

Financial & competing interests disclosure

This study received funding from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES; MCS: No. 201567/2009-1; AC Anauate, CO Gigek); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq; MCS: No. 303871/2014-8; RR Burbano); and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP; MCS: No. 2016/25562-, MFL: No. 2012/14768-5, FW: No. 2016/19953-6). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations. In addition, for investigations involving human subjects, informed consent has been obtained from the participants involved.