https://orcid.org/0000-0002-3314-9704
Abstract
Aim: To investigate whether kaempferol exhibits a protective effect on high glucose-induced epithelial–mesenchymal transition (EMT) by mediating the PVT1/miR-214 and PAK4/β-catenin pathways in SRA01/04 cells. Methods & methods: qRT-PCR and western blot assays were used for gene and protein determination, and migration and invasion assays were conducted. A coimmunoprecipitation assay was used for determining protein interactions. Results: High glucose effectively upregulated PVT1 expression, downregulated miR-214 expression and promoted cell migration and invasion. Kaempferol attenuated high glucose-induced EMT by increasing PVT1 expression and decreasing miR-214 expression. PAK4 was identified as a direct target of miR-214. PAK4 overexpression could rescue the effects of PVT1 deficiency on SRA01/04 cells. Conclusion: Kaempferol ameliorated the regulatory effects of PVT1/miR-214 on high glucose-induced EMT through PAK4/β-catenin in SRA01/04 cells.
Financial & competing interests disclosure
This study was financially supported by the Science and Technology Program for Social Development in Kunshan (KS1937) and the National Natural Science Foundation of China (81960883, 82060407). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.
No writing assistance was utilized in the production of this manuscript.
Data availability
The data used to support the findings of this study are included within the article.