Purification of heat labile toxin from Bordetella pertussis vaccine strain 134 employed indigenous technology

Peer review under responsibility of Alexandria University Faculty of Medicine.

Available online 23 June 2015

Figures & data

Figure 1 SDS–PAGE analysis of DEAE purified B. pertussis (134).

Table 1 Total protein analysis.

S. no.	Sample	OD value (280 nm)	Protein concentration (μg)
1	Crude (neat)	1.245	207.5
2	Crude (1:10)	0.160	26.66
3	TCA(s)	1.635	272.5
4	TCA(p)	0.028	4.67

Table 2 Bed height analysis for the optimal recovery of pertussis protein.

S. no.	Height of the gel (cm)	Peak analysis	OD 280 nm	Total protein concentration (μg)
1	2	Asymmetry	1.402	233.6
2	4	Symmetry	1.802	300.3
3	6	Symmetry	1.902	317
4	8	Asymmetry	2.102	350

Figure 2 Analysis DEAE purified B. pertussis fractions.

Figure 3 ODD assay for DEAE purified B. pertussis (134).

Figure 4 Ischemic inducing activity of guinea pig after 24 h.