Evaluation of Interleukin 8 gene polymorphism for predicting inflammation in Indian chronic kidney disease and peritoneal dialysis patients

Peer review under responsibility of Alexandria University Faculty of Medicine.

Available online 9 July 2016

Figures & data

Table 1 Clinical parameters for study population.

Parameters	Diabetic CKD	CAPD	Control
Age in years	61.37 ± 12.98	58.2 ± 9.78	56.54 ± 11.9
BMI	23.96 ± 3.06	21.63 ± 4.18	20.93 ± 4.73
Bl.Glucose (mg/dl)	180.45 ± 36.03	127.83 ± 11.62	118.86 ± 11.17
Bl.Urea (mg/dl)	99.6 ± 48	91. 11 ± 53.20	22.1 ± 15.9
S.Creatinine (mg/dl)	4.60 ± 2.45	5.9 ± 1.29	1.32 ± 0.62
S.Sodium (meq/dl)	135.85 ± 10.98	137.66 ± 9.50	139 ± 5.95
S.Potassium (meq/dl)	3.96 ± 1.97	4.36 ± 1.75	4.1 ± 0.95
S.CRP (mg/dl)	6.48 ± 3.47	6.80 ± 2.83	1.06 ± 0.88
Total cholesterol (mg/dl)	241.63 ± 37.83	239.10 ± 27.53	192.88 ± 33.71
Triglycerides (mg/dl)	224.20 ± 23.19	218.81 ± 21.67	167.04 ± 32.0
HDL (mg/dl)	27.6 ± 5.6	26.4 ± 4.93	34.5 ± 4.3
LDL (mg/dl)	186.4 ± 29.12	187.9 ± 31.50	146.72 ± 22.61

Figure 1 Genomic DNA isolation from blood of cases and controls. Lane 1 – marker (Lambda DNA/EcoRI digest), Lane 3 – blood genomic DNA (group I), Lane 4 – blood genomic DNA (group II), Lane 5 – blood genomic DNA (group III).

Figure 2 PCR amplification of IL-8 gene in cases and controls. Lane 1 – marker (100 bp ladder), Lane 3 – PCR product (group I), Lane 4 – PCR product (group II), Lane 5 – PCR product (group III).

Figure 3 Restriction digestion of PCR products of IL-8 gene in cases and controls for detection of +781 C/T polymorphism. Lane 1 – marker (100 bp ladder), Lane 2 – EcoRI digested PCR product (group I), Lane 3 – EcoRI digested PCR product (group II), Lane 4 – EcoRI digested PCR product (group III).

Table 2 Distribution of IL-8 genotypes and allele frequencies in study groups.

Study group	CC	CT	TT	C	T
Control	18	24	8	0.6	0.4
CAPD	13	25	12	0.51	0.49
CKD	9	21	20	0.39	0.61

Table 3 Comparison of genotype and allelic frequency distribution in study groups.

Study groups	Chi-square value	Degree of freedom	p-value	Odds ratio	95% Confidence interval
Control vs CAPD	1.627	2	<0.05 (0.443)	1.083	0.4945–2.3735
Control vs CKD	8.343	2	<0.05 (0.015)	0.785	0.3562–1.7276