Effect of immunomodulator, fingolimod, on ischemia reperfusion testicular injury in rats: Targeting the role of sphingolipid rheostat (fingolimod on I/R induced testicular injury)

Peer review under responsibility of Alexandria University Faculty of Medicine.

Available online 25 July 2016

Figures & data

Figure 1 Effect of FTY720 treatment on antioxidant enzyme activities in testicular tissue (A) testicular GSH-Px, (B) testicular SOD, and (C) testicular catalase. The mean (horizontal line) and individual values are shown in each experimental group. Values are mean ± SD. ^*P < 0.05 vs. sham group, ^**P < 0.05 vs. I/R group.

Table 1 Effect of FTY720 treatment on antioxidant enzyme activities in testicular tissue and testicular MDA.

Parameter	Sham group	I/R group	I/R+FTY720 group
Testicular GSH-Px (U/gm)	3.62 ± 0.9	10.69 ± 1.823^⁎	3.56 ± 0.506^⁎⁎
Testicular SOD (U/mg)	0.04 ± 0.006	0.139 ± 0.008^⁎	0.069 ± 0.01^⁎⁎
Testicular Catalase (U/gm)	0.192 ± 0.005	0.180 ± 0.003^⁎	0.194 ± 0.005^⁎⁎
Testicular MDA (nmol/gm)	1.22 ± 0.052	1.36 ± 0.0299^⁎	1.24 ± 0.056^⁎⁎

Glutathione peroxidase (GSH-Px), Superoxide dismutase (SOD) and Malondialdehyde (MDA).

⁎ P < 0.05 versus sham group.

⁎⁎ P < 0.05 versus I/R group. All values are expressed as mean ± SD of 10 rats in each group.

Figure 2 Effect of FTY720 treatment on testicular MDA. Values are mean ± SD. ^*P < 0.05 vs. sham group, ^**P < 0.05 vs. I/R group.

Figure 3 Effect of FTY720 treatment on testicular sphingolipids (A) testicular sphingomyelin, (B) testicular sphingosine-1 phosphate, and (C) testicular total ceramide. Values are mean ± SD. ^*P < 0.05 vs. sham group, ^**P < 0.05 vs. I/R group.

Figure 4 Testicular histopathology (A): Normal testicular tissue in sham group (B):Testicular tissue in I/R group (C): Testicular tissue in I/R+FTY720 group.

Table 2 Effect of FTY720 treatment on Johnsen’s score.

Groups	Johnsen’s score
Sham group	9.37 ± 0.37
I/R group	6.77 ± 0.71^⁎
I/R+FTY720 group	8.83 ± 0.62^⁎⁎

⁎ P < 0.05 versus sham group.

⁎⁎ P < 0.05 versus I/R group. All values are expressed as mean ± SD of 10 rats in each group.