Effect of Prosthechea karwinskii (Orchidaceae) on obesity and dyslipidemia in Wistar rats

Peer review under responsibility of Alexandria University Faculty of Medicine.

Available online 2 January 2017

Figures & data

Table 2 Caloric intake and body weight of rats under the effect of P. karwinskii extracts after 13 days of experimental treatment.

Parameter	CG	MS	P	L	F
kcal/day (B)	65.10 ± 19.53	125.68 ± 22.62^a	87.43 ± 2.38	91.27 ± 10.38	81.30 ± 9.26
Initial weight (g)	372.0 ± 70.14	440.0 ± 83.19	321.67 ± 96.3	385.0 ± 69.28	431.67 ± 23.63
kcal/day (E)	79.05 ± 6.48	139.42 ± 6.77^a	128.35 ± 6.47^a	147.93 ± 31.13^a	147.78 ± 13.96^a
Final weight (g)	376.0 ± 77.73	464.50 ± 44.04	296.67 ± 88.8	365.0 ± 73.65	410.0 ± 20.00

CG: control group, MS: sucrose diet, P: sucrose diet and pseudobulb extract, L: sucrose diet and leaf extract, F: sucrose diet and flower extract, B: Beginning of the treatment, E: End of the treatment. The values represent the mean ± SD, superscript values show statistically significant difference as revealed by the Tukey’s test.

a P < 0.05.

Table 1 Antioxidant capacity of Prosthechea karwinskii extracts.

Extract	Yield w/w (%)	IC50	AAI
Pseudobulb	18.8 ± 1.335	43.06 ± 7.311	0.925 ± 0.162
Leaf	16.8 ± 2.335	6.91 ± 0.187	5.7 ± 0.157
Flower	33.6 ± 2.93	30.85 ± 2.51	1.276 ± 0.971

IC₅₀: 50% inhibitory concentration. AAI: antioxidant activity index = [DPPH]/IC₅₀.

Fig. 1 Caloric intake during Metabolic Syndrome development. CG: control group, MS: sucrose diet, kcal: kilocalories. The values represent the mean ± DS ^∗P < 0.05.

Table 3 Abdominal, epididymal and pericardial adipose tissue weight and adiposity index after co-treatment with P. karwinskii extracts.

Parameter (g)	GC	MS	P	L	F
AT abdominal	12.20 ± 1.92	16.25 ± 5.91	9.33 ± 3.78^a	11.66 ± 2.30	11.0 ± 1.73^a
AT epididymal	10.0 ± 2.55	15.83 ± 6.67	6.66 ± 2.08^a	8.33 ± 2.88^a	10.33 ± 0.57^a
AT pericardial	0.62 ± 0.40	0.48 ± 0.18	0.70 ± 0.26	0.56 ± 0.30	0.40 ± 0.10
Total fat	22.82 ± 4.87	32.56 ± 12.76	16.69 ± 6.12^a	20.55 ± 5.48^a	21.73 ± 2.4^a

AT: adipose tissue, GC: control group, MS: sucrose diet, P: sucrose diet and pseudobulb extract, L: sucrose diet and leaf extract, F: sucrose diet and flower extract. The values represent the mean ± SD, superscript values show statistically significant difference as revealed by the Tukey’s test.

a P < 0.05.

Table 4 Variation of serum parameters in Wistar rats after 13 days of treatment with P. karwinskii extracts.

Parameter (mg/dl)	CG	MS	P	L	F
Glucose	96.80 ± 10.64^a	135.26 ± 30.70	92.20 ± 7.95^a	64.12 ± 11.39^a	73.72 ± 25.76^a
Cholesterol	69.43 ± 10.39^a	87.52 ± 7.44	75.55 ± 0.32^a	76.04 ± 9.59^a	53.89 ± 10.54^a
Triglycerides	214.28 ± 18.07^a	259.28 ± 23.89	127.75 ± 46.56^a	108.78 ± 11.30^a	74.58 ± 22.35^a

CG: control group, MS: sucrose diet, P: sucrose diet and pseudobulb extract, L: sucrose diet and leaf extract, F: sucrose diet and flower extract. The values represent the mean ± SD, superscript values show statistically significant difference as revealed by the Tukey’s test.

a P < 0.05.