Comparison of immunogenical potency of Leishmania major (MRHO/IR/75/ER) antigens prepared by 3 different methods in conjunction with Alum-Naltrexone adjuvant in BALB/c mice

Peer review under responsibility of Alexandria University Faculty of Medicine.

Available online 7 December 2018

Figures & data

Table1 Different groups specified subcutaneous injection doses.

Injections
Group name	SLA (µl)	FKP (µl)	ALM (µl)	Nalt. (µl)	ALUM (µl)	PBS (µl)
SLA-Alum-Nalt.	50	–	–	50	50	–
FKP-Alum-Nalt.	–	50	–	50	50	–
ALM-Alum-Nalt.	–	–	50	50	50	–
ALM	–	–	50	–	–	100
SLA	50	–	–	–	–	100
FKP	–	50	–	–	–	100
Control	–	–	–	–	–	150

Fig. 1 Effect of immunization of mice with ALM, FKP or SLA antigens with or without ALUM-NALT mixture as adjuvant on splenic lymphocytes proliferation on exposure to ALM antigen. (^*: p value < 0.05/^**: p value < 0.01/^***: p value < 0.001).

Fig. 2 Effect of immunization of mice with ALM, FKP or SLA antigens with or without ALUM-NALT mixture as adjuvant on the splenic IFN-γ production on exposure to ALM antigen. (^*: p value < 0.05/^**: p value < 0.01/^***: p value < 0.001).

Fig. 3 Effect of immunization of mice with ALM, FKP or SLA antigens with or without ALUM-NALT mixture as adjuvant on the splenic IL-5 production on exposure to ALM antigen. (^*: p value < 0.05/^**: p value < 0.01/^***: p value < 0.001).

Fig. 4 Comparison of optical densities from ELISA test for serum anti-ALM-specific total IgG among different groups of mice. (^*: p value < 0.05/^**: p value < 0.01/^***: p value < 0.001).

Fig. 5 Comparison of optical densities from ELISA test for serum anti-ALM-specific IgG1 among different groups of mice. (^*: p value < 0.05/^**: p value < 0.01/^***: p value < 0.001).

Fig. 6 Comparison of optical densities from ELISA test for serum anti-ALM-specific IgG_2a among different groups of mice. (^*: p value < 0.05/^**: p value < 0.01/^***: p value < 0.001).