Evaluation of antioxidant, antimicrobial and cytotoxic activities of seed crude extracts of Ammi majus grown in Oman

Figures & data

Table 1 Antioxidant activity of different polarities seed crude extracts of A. majus.

Seed crude extracts	Conc µg/mL	Log C	% Inhibition	IC50 µg/mL
Hexane	12.5	1.096	55.91 ± 0.10
	25	1.397	47.92 ± 0.17
	50	1.698	45.36 ± 0.31	123.651
	100	2	43.45 ± 0.16
	200	2.301	32.26 ± 0.12
Ethyl acetate	12.5	1.096	53.99 ± 0.18
	25	1.397	47.92 ± 0.15
	50	1.698	44.08 ± 0.33	236.246
	100	2	34.82 ± 0.54
	200	2.301	22.68 ± 0.87
Chloroform	12.5	1.096	56.23 ± 0.23
	25	1.397	52.07 ± 0.24	55.97
	50	1.698	67.73 ± 0.10
	100	2	50.79 ± 0.75
	200	2.301	38.01 ± 0.08
Butanol	12.5	1.096	48.88 ± 0.18
	25	1.397	51.75 ± 0.10
	50	1.698	46.96 ± 0.16	139.958
	100	2	43.45 ± 0.14
	200	2.301	28.11 ± 0.19
Methanol	12.5	1.096	48.88 ± 0.18
	25	1.397	46.96 ± 0.25
	50	1.698	50.79 ± 0.34	80.164
	100	2	41.21 ± 0.73
	200	2.301	49.84 ± 0.29
Water	12.5	1.096	54.95 ± 0.12
	25	1.397	56.86 ± 0.17
	50	1.698	55.59 ± 0.39	55.207
	100	2	53.35 ± 0.56
	200	2.301	43.13 ± 0.87

Values are given as mean ± SD (n = 3).

Table 2 Antimicrobial activity of different polarities seed crude extracts of A. majus.

Seed crude extracts	Conc mg/mL	H. influenza (mm)	S. aureus (mm)	E. coli (mm)	Proteus
Methanol	Control	nd	10 ± 0.12	9 ± 0.17	6 ± 0.13
	2	nd	8 ± 0.17	11 ± 0.10	9 ± 0.16
	1	nd	7 ± 0.26	15 ± 0.17	7 ± 0.20
	0.5	nd	6 ± 0.15	12 ± 0.32	7 ± 0.54
	0.25	nd	7 ± 0.72	9 ± 0.09	9 ± 0.32
Hexane	Control	7 ± 0.10	10 ± 0.17	25 ± 0.09	13 ± 0.15
	2	8 ± 0.41	11 ± 0.13	10 ± 0.08	9 ± 0.17
	1	8 ± 0.18	13 ± 0.12	12 ± 0.12	9 ± 0.12
	0.5	7 ± 0.10	12 ± 0.15	9 ± 0.19	10 ± 0.12
	0.25	7 ± 0.11	14 ± 0.17	8 ± 0.13	10 ± 0.10
Chloroform	Control	7 ± 0.72	7 ± 0.25	24 ± 0.44	7 ± 0.17
	2	9 ± 0.16	9 ± 0.20	12 ± 0.10	8 ± 0.18
	1	8 ± 0.23	8 ± 0.42	10 ± 0.11	8 ± 0.12
	0.5	8 ± 0.53	10 ± 0.10	10 ± 0.17	8 ± 0.15
	0.25	7 ± 0.16	10 ± 0.09	8 ± 0.12	7 ± 0.12
Ethyl acetate	Control	6 ± 0.18	9 ± 0.13	24 ± 0.10	nd
	2	7 ± 0.09	8 ± 0.17	10 ± 0.42	nd
	1	6 ± 0.12	8 ± 0.18	10 ± 0.45	nd
	0.5	7 ± 0.14	9 ± 0.12	12 ± 0.23	nd
	0.25	7 ± 0.10	9 ± 0.15	10 ± 0.34	nd
Butanol	Control	7 ± 0.23	10 ± 0.12	23 ± 0.56	6 ± 0.10
	2	8 ± 0.43	9 ± 0.15	7 ± 0.23	9 ± 0.18
	1	8 ± 0.34	10 ± 0.10	7 ± 0.19	9 ± 0.22
	0.5	6 ± 0.67	14 ± 0.11	10 ± 0.10	8 ± 0.27
	0.25	7 ± 0.27	12 ± 0.43	10 ± 0.12	9 ± 0.13
Water	Control	9 ± 0.10	11 ± 0.35	22 ± 0.15	8 ± 0.09
	2	9 ± 0.19	7 ± 0.44	9 ± 0.10	8 ± 0.15
	1	7 ± 0.13	8 ± 0.17	8 ± 0.12	9 ± 0.18
	0.5	8 ± 0.11	8 ± 0.08	9 ± 0.18	8 ± 0.12
	0.25	8 ± 0.56	9 ± 0.12	10 ± 0.14	8 ± 0.19

nd, not detectable inhibition zone. Values are given as mean ± SD (n = 3).

Table 3 Percentage of mortality and lethal concentration (LC₅₀) of different polarities seed crude extracts of A. majus.

Seed crude extracts	Conc µg/mL	Mortality (%)	LC50 (µg/mL)
Hexane	1000	100
	500	100
	100	60	50.818
	10	20
	Control	0
Ethyl acetate	1000	100
	500	10	275.021
	100	40
	10	20
	Control	0
Chloroform	1000	100
	500	40	49.168
	100	30
	10	20
	Control	0
Butanol	1000	90
	500	20	312.982
	100	10
	10	10
	Control	0
Methanol	1000	100
	500	40	191.52
	100	50
	10	20
	Control	0
Water	1000	100
	500	20	652.382
	100	10
	10	10
	Control	0

Values are given as mean ± SD (n = 3).