Correlation between iron regulatory protein-1 (G-32373708A) and -2 (G-49520870A), gene variations and migraine susceptibility in southeast Iran: A case-control study

Figures & data

Table 1 Demographic distribution of migraine patients and control subjects enrolled in the study.

Characteristics	Control (n = 200) N (%)	Case (n = 190) N (%)
Gender (male/female)	68(34)/132(66)	42(22)/148(78)
Age (year) < 50	174(87)	180(95)
Age (year) ≥ 50	26(13)	10(5)
Mean ± SD Range	35.1 ± 12.2 15–75	31.7 ± 10.17 13–66
Smoking (yes/no)	80(40)/120(60)	88(46)/102(54)
Race (parse/other)	144(72)/56(28)	146(77)/44(23)
Migrate (yes/no)	96(48)/104(52)	72(38)/118(62)

Table 2 Risk of migraine in association with gender, age, smoking, race and migration.

Characteristics	Control (n = 200) N (freq.)	Case (n = 190) N (freq.)	OR (95%CI)	P-value
Gender
Males	68(0.34)	42(0.22)	0.55(0.291–1.042)	0.067
Females	132(0.66)	148(0.78)	1.81(0.960–3.433)	0.067
Age (year)
Age < 50	174(0.87)	180(0.95)	2.69(0.92–7.862)	0.071
Age ≥ 50	26(0.13)	10(0.05)	0.37 (0.127–1.08)	0.071
Smoking
Ever (current, former)	80(0.40)	88(0.46)	1.29(0.73–2.28)	0.37
Never	120(0.60)	102(0.54)	0.77(0.438–1.364)	0.37
Race
Persian	142(0.71)	146(0.77)	1.35(0.712–2.579)	0.35
Other	58(0.29)	44(0.23)	0.73(0.388–1.404)	0.35
Migrate
Yes	96(0.48)	72(0.38)	0.66(0.374–1.170)	0.15
No	104(0.52)	118(0.62)	1.51(0.855–2.677)	0.15

Comparisons were performed by independent samples t-test Χ ²of test results for categorical data, freq. = frequency, OR = odds ratio, CI = confidence interval, N = number.

Table 3 Distribution of genotypes and frequency of alleles of the g.32373708 G/A-IRP-1 polymorphisms in patients with migraine and individuals without disease (controls).

IRP-1 (rs867469)	Control (n = 200)	Case (n = 190)	Crude OR (95%CI)	Crude P-value	Adjusted. OR^a (95% CI)	Adjusted. P-value^a
AA, No (%)	20(10)	20(10.5)	1	–	1	–
AG, No (%)	162(81)	110(58)	0.67(0.265–1.740)	0.421	0.63(0.242–1.642)	0.345
GG, No (%)	18(9)	60(31.5)	3.33(1.055–10.530)	0.040	3.82(1.131–12.953)	0.031
	P = <0.001
A allele	101(50.5)	75(39.5)	1	-	1	–
G allele	99(49.5)	115(60.5)	1.56(1.047-2.338)	0.029	1.61(1.068-2.428)	0.023
	P = 0.029

CI: confidence interval, OR: odds ratio.

Significant P-values are in bold.

a Adjusted for age and sex.

Table 4 Distribution of genotypes and frequency of alleles of the g.49520870 G/A-IRP-2 polymorphisms in patients with migraine and individuals without disease (controls).

IRP-2 (rs17483548)	Control (n = 200)	Case (n = 190)	Crude OR (95%CI)	Crude P-value	Adjusted. OR^a (95% CI)	Adjusted. P-value^a
AA, No (%)	16(8.0)	12(6.3)	1	–	1	–
AG, No (%)	154(77.0)	130(68.4)	2.11(0.77–5.752)	0.144	0.63(0.242–1.642)	0.133
GG, No (%)	30(15.0)	48(25.3)	7.5(2.171–25.906)	0.001	19.12(3.69–99.05)	0.001
	P = 0.002
A allele	92(46.0)	71(37.4)	1	–	1	–
G allele	108(54.0)	119(62.6)	1.42(0.953–2.140)	0.085	1.51(0.998–2.288)	0.051
	P = 0.08

CI: confidence interval, OR: odds ratio.

Significant P-values are in bold.

a Adjusted for age and sex.

Table 5 Distribution of genotypes and frequency of alleles of the g.32373708 G/A-IRP-1 and g.49520870 G/A-IRP-2 polymorphisms in patients with different migraine subtypes.

IRP-1 (rs867469)	With aura, No (%) (n = 78)	Without aura, No (%) (n = 112)	OR(95%CI)	P-value
AA, No (%)	8(10.3)	12(10.7)	1	–
AG, No (%)	34(43.6)	76(67.9)	1.49(0.372–5.973)	0.573
GG, No (%)	36(46.2)	24(21.4)	0.44(0.103–1.915)	0.277
	P = 0.033
IRP-2 (rs17483548)
AA, No (%)AG, No (%)GG, No (%)	8(7.1)	4(5.1)	1	–
	96(85.7)	34(43.6)	1.41(0.237–8.416)	0.705
	8(7.1)	40(51.3)	0.10(0.013–0.745)	0.025
	P < 0.001

CI: confidence interval, OR: odds ratio. Significant P-values are in bold.