Figures & data
Fig. 1 Free radical scavenging capacity of Natural extracts. The colour code is as follows: 1 Blue: Superoxide free radicals (OD at 560 nm), 2 Red: Total antioxidant (OD at 765 nm), 3 Green: Hydroxyl free radicals (OD at 532 nm), for different estimations.
Fig. 2 Cell proliferation in breast cancer cells with NE mix and Tamoxifen treatment. Number of proliferating cells (Average ± SE) is shown as assessed by MTT (3-(4, dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide) colour formation following the treatment with different concentrations of NE extracts mix and Tamoxifen (Tam). Two way ANOVA followed by student T-test was used for statistical analysis and significance between the groups and the difference was considered significant for P values <0.05 (*P < 0.05, **P < 0.005).
Fig. 4 Apoptotic cell death in breast cancer cells with NE mix and Tamoxifen treatment. Percentage of apoptotic cells (Average ± SE) as assessed by Annexin-V-Alexa Fluor 488 binding, following the treatment with different concentrations of extracts NE mix and Tamoxifen (Tam). Two way ANOVA and student’s T-test were used to test for statistical significance between the groups and the difference was considered significant for p values <0.05*.
Fig. 5 Apoptosis in breast cancer and melanoma cells treated with NE mix and Tamoxifen. Percentage of PI-positive (Average ± SE) in cells after treatment with different concentrations of extracts NE mix, and Tam. One way ANOVA was used to test for statistical significance between the groups and the difference was considered significant for P values <0.05 (*P < 0.05, **P < 0.005, ***P < 0.001).
Fig. 6 a and b: For fluorescent imaging of apoptotic cells, cells were labelled with 5 µl of Annexin-V-Alexa Fluor 488 and 1 µl of propidium iodide and incubated for 20 min at 37 °C. EVOS Digital inverted fluorescence microscope (Invitrogen) with a 10X LPlanFL PH fluorescence objective was used to image the labelled cells in the plate.
Fig. 7 Gene expression of apoptotic markers in immortal cell lines (MCF-7, ZR-75, MDA MB-231) when treated with NE mix and Tamoxifen individually and in combination.
Fig. 8 a–c: The positive ion LC-MS/MS spectra of standards of curcumin, turmeric, ginger and garlic.
