Visfatin gene expression and oxidative stress in pregnancy induced hypertension

Figures & data

Table 1 The sequence of primers used in real-time PCR.

Oligo name	Len	Sequence
GAPDH-F	20	5′-CTC TGC TCC TCC TGT TCG AC-3′
GAPDH-R	20	5′-GCG CCC AAT ACG ACC AAA TC-3′
NAMPT-F	24	5′-GAC GCC AGC AGGGAA TTT TGT TAC-3′
NAMPT-R	24	5′-AGC TTT TTG TCA CCT TGC CAT TCT-3′

Table 2 The placental visfatin gene expression in hypertensive pregnant women groups compared to normotensive pregnant women group.

	Groups
	Normalized group	Hypertensive Groups (n = 80)
Parameter		Gestational	Mild preeclampsia	Severe preeclampsia	Chronic with superimposed
Placental visfatin gene expression Mean ± SD n P	1.56 ± 0.69 10	1.28 ± 0.42 20 0.254	1.01 ± 0.24 16 0.034^*	0.40 ± 0.14 25 <0.001^**	0.32 ± 0.11 19 <0.001^**

P-Value compared with control group. *Significant p < .05. **Highly significant p < .001.

Table 3 The oxidative stress measured parameters in hypertensive compared to normotensive pregnant women groups.

	Groups
	Normalized group (n = 10)	Hypertensive (n = Groups 80)
Parameter		Gestational (n = 20)	Mild preeclampsia (n = 16)	Severe preeclampsia (n = 25)	Chronic with superimposed (n = 19)
Serum CAT (U/L) Mean ± SD P	853.74 ± 85.16	805.76 ± 84.790.156	687.33 ± 211.030.027^*	588.33 ± 175.55 < 0.001^**	518.56 ± 185.40 < 0.001**
Serum TAC (mM/L) Mean ± SD P	0.96 ± 0.13	0.88 ± 0.140.152	0.76 ± 0.150.002^*	0.60 ± 0.18 < 0.001^**	0.49 ± 0.16 < 0.001**
Blood GSH (mg/dl) Mean ± SD P	2.14 ± 0.81	1.78 ± 0.540.157	1.45 ± 0.310.026^*	0.79 ± 0.24 < 0.001^**	0.72 ± 0.17 < 0.001^**
Serum MDA (nmol/ml) Mean ± SD P	4.32 ± 1.34	4.96 ± 1.280.218	6.47 ± 1.590.002^*	7.96 ± 1.91 < 0.001^**	8.88 ± 1.19 < 0.001^**

P-Value compared with control group. ^*Significant p < .05. ^**Highly significant p < .001.

Fig. 1 Linear Pearson correlation between each serum CAT and serum MDA among mild preeclampsia pregnant women group (n = 16).

Fig. 2 Linear Pearson correlation between each serum CAT and serum TAC among severe preeclampsia pregnant women group (n = 25).

Fig. 3 Linear Pearson correlation between each serum TAC and tissue visfatin gene among chronic hypertension with superimposed preeclampsia pregnant women group (n = 19).