Anti-cancer potential of the lipoidal and flavonoidal compounds from Pisum sativum and Vicia faba peels

Figures & data

Table 1 GC/MS analysis of the n-hexane extract of P. sativum:

Compound	Mol.	BP	Relative
	Weight		area %
n-Dodecane	170	43	3.61
n-Tridecane	184	43	4.75
Dodecanol	186	41	2.31
2,2dimethyl-1-(2,4,6trimethylphenyl) Propan- 1-one	204	147	12.56
Di-t-butylphenol	206	191	4.35
Tetradecanol	214	41	1.77
(4E,8E)5-Propyl trideca-4,8-dien-6-yne	218	91	2.77
2-Phenyl Decane	218	105	3.89
6-Phenylundecane	232	91	12.18
6-Phenyldodecane	246	91	10.63
n-Hexadecanoic acid	256	43	10.35
6-Phenyl tridecane	260	91	7.12
2- Phenyl tridecane	260	105	0.81
8,9-Dihydrocyclohept phenalen-7,10-dione	260	91	0.86
Octadecanol	270	41	2.80
n-Docosane	310	43	0.28
Pregn-4-ene-3,20-dione	314	124	0.20
9-n- hexyl heptadecane	324	43	0.28
Tetracosane	338	57	0.46
4-Phenyl Eicosane	358	91	1.01
Hydrocarbons			47.79
Fatty alcohols			11.23
Ketones			13.62
Acids			10.35
Total identified			82.99

Table 2 GC/MS analysis of the n-hexane extract of V. faba.

Compound	Mol.	BP	Relative
	Weight		area %
n-Decane	142	57	0.68
Nonanal	142	41	3.67
Decanol	158	41	1.12
Dodecanol	186	41	0.74
3-Methyl pentenyl phenyl ketone	188	161	2.61
7-phenyl Tridecane	218	91	6.03
Pentadecanol	228	41	3.91
5-phenylundecane	232	91	23.24
2 (Benzoyloxy)cycloheptanone	232	105	12.74
n-Heptadecane	240	57	2.61
Hexadecanol	242	41	3.65
7,7-Diphenyl-2,4,6-heptatrienal	260	260	8.91
Methyl palmitate	270	74	6.24
n-Eicosene	280	41	2.24
Methyl linoleate	294	67	5.56
n-Heneicosane	296	57	0.90
α, α-Carotene4,4′-dione	564	83	1.12
Hydrocarbons			35.70
Fatty alcohols			9.42
Aldehydes			12.58
Ketones			16.47
Esters			11.80
Total identified			85.97

Table 3 HPLC analysis of flavonoids in the ethyl acetate fractions of P. sativum and V. faba.

Flavonoids	Concentration (mg/100 g)
	Pisum sativum	Vicia faba
Apigenin-6-arabinose-8-galactose	170.01	68.46
Apigenin-6-rhamnose-8-glucose	357.13	185.53
Naringin	201.41	109.79
Hesperidin	605.94	245.85
Rutin	83.01	030.24
Apigenin-7-O-neohespiroside	104.13	027.30
Kaempferol-3,7-dirhamnoside	013.26	008.20
Quercetrin	256.26	–
Apigenin-7-glucose	147.59	015.48
Acacetin-7-neo hesperside	136.87	043.28
Acacetin neo rutinoside	061.77	073.71
Quercetin	056.90	095.20
Naringenin	024.59	071.98
Hespirtin	158.29	112.28
Kaempferol	019.79	016.61
Rhamentin	248.32	070.12
Apigenin	014.31	056.41

Table 4 HPLC analysis of phenolics in the ethyl acetate fractions of P. sativum and V. faba.

Phenolic compounds	Concentration (ppm)
	Pisum sativum	Vicia faba
Gallic acid	218.45	15.44
Pyrogallol	860.06	850.38
4-Amino benzoic acid	069.25	012.63
Protocatchuic acid	426.15	400.36
Catechein	795.74	599.65
Chlorogenic acid	742.28	197.24
Catechol	852.94	158.36
Caffeine	656.52	194.47
ρ-Hydroxy benzoic acid	588.94	810.02
Caffeic acid	146.11	106.54
Vanillic acid	536.67	–
ρ-Coumaric acid	462.93	150.76
Ferulic acid	788.29	609.38
Iso-Ferulic acid	210.39	076.25
Ellagic acid	433.87	–
Alpha coumaric acid	–	066.95
Benzoic acid	492.22	737.22
3,4,5-Methoxycinnamic acid	477.43	213.31
Cinnamic acid	206.97	219.50

Fig. 1 The antioxidant activities of the four extracts, in vitro, using the DPPH• assay.

Fig. 2 Screening of the four extracts on breast carcinoma (MCF-7) and colon carcinoma (HCT-116).

Fig. 3 Screening of the 4 compounds on breast carcinoma (MCF-7) and colon carcinoma (HCT-116), the results expressed by % of inhibition at 100 ug/ml.

Fig. 4 BcL 2 quantitative analysis, where Y axis represents The protein levels (ng/ml).

Fig. 5 Bax quantitative analysis, where Y axis represents the protein levels (ng/ml).

Fig. 6 Bax-Bcl2 ratio.

Fig. 7 Caspase 7 protein level (ng/ml) in treated breast cancer cells and untreated compared to doxorubicin.