Ontogenic development of immunoglobulins (Igs)-positive lymphocytes in the lymphoid organs of native chickens of Bangladesh

Peer review under responsibility of Faculty of Veterinary Medicine, Cairo University.

Figures & data

Figure 1 (A) The frequency of Igs-positive lymphocytes in the bursa of Fabricius from ED 8 to ED 20. The present graph shows that, IgM-positive lymphocytes are first detected in bursa of Fabricius on ED 10. The number of IgM-positive lymphocytes in bursa of Fabricius are increased significantly (p < 0.001) from the ED 14 to the ED 20 comparing with ED 10. IgG-positive lymphocytes are observed only on ED 20. No IgA-positive lymphocytes are present. Values are given in mean ± SEM (n = 6). (B) Immunostained sections of bursa of Fabricius of chicken’s embryo at ED 20. The IgM-positive lymphocytes (arrows) are present uniformly in cortex and medulla. C, cortex; M, medulla. Scale bar = 7.5 μm.

Figure 2 (A) The frequency of Igs-positive lymphocytes in the spleen from ED 8 to ED 20. The present graph shows that IgM-positive lymphocytes are first detected in spleen on ED 14. The number of IgM-positive lymphocytes in spleen are increased significantly (p < 0.001) on ED 20 comparing with ED 14. IgG-positive lymphocytes are observed only on ED 20. No IgA-positive lymphocyte is present. Values are given in mean ± SEM (n = 6). (B) Immunostained sections of the chicken’s embryonic spleen at ED 20. The IgM-positive cells (arrows) are present mainly around the white pulps. Scale bar = 7.5 μm.

Figure 3 (A) The frequency of Igs-positive lymphocytes in the thymus from ED 8 to ED 20. The present graph shows that only IgM-positive lymphocytes are detected in spleen on ED 20. No IgG- and IgA-positive lymphocytes are observed. Values are given in mean ± SEM (n = 6). (B) Immunostained sections of thymus of chicken’s embryo at ED 20. The cortex of thymus shows more IgM-positive lymphocytes (arrows) than the medulla. C, cortex; M, medulla. Scale bar = 7.5 μm.

Figure 4 (A) The frequency of Igs-positive lymphocytes in the cecal tonsil from ED 8 to ED 20. The present graph shows that only IgM-positive lymphocytes are detected in cecal tonsil on ED 20. No IgG- and IgA-positive lymphocytes are observed. Values are given in mean ± SEM (n = 6). (B) Immunostained sections of the cecal tonsil of chicken’s embryo at ED 20. The lamina propria of cecal tonsils shows very few IgM-positive lymphocytes (arrows). Scale bar = 7.5 μm.

Figure 5 (A) The comparative frequency of IgM-positive lymphocytes in cecal tonsil, thymus, spleen and bursa of Fabricius from ED 14 to ED 20. The present graph shows the IgM-positive lymphocytes are significantly (p < 0.001) higher in bursa of Fabricius than that of other lymphoid organs at all stages from ED14 to ED20. Values are given in mean ± SEM (n = 6). (B) The comparative frequency of IgG-positive lymphocytes in cecal tonsil, thymus, spleen and bursa of Fabricius on ED 20. The present graph shows the IgG-positive lymphocytes are significantly (p < 0.005) higher in bursa of Fabricius than that of other lymphoid organs. Values are given in mean ± SEM (n = 6).