Antidotal impact of extra virgin olive oil against genotoxicity, cytotoxicity and immunotoxicity induced by hexavalent chromium in rat

Peer review under responsibility of Faculty of Veterinary Medicine, Cairo University.

Figures & data

Figure 1 Micronucleus containing erythrocytes of control (A) and K₂Cr₂O₇ receiving rats (0.4 mg/kg bw/day) (B).

Figure 2 Effects of oral EVOO administration (300 μl) and K₂Cr₂O₇ (0.4 mg/kg bw/day) on micronucleus frequency (A), serum levels of 8 OHdG (B) and LDH activity (C). Each bars carrying different letters were significantly different (P < 0.05) (mean ± SE, n = 6).

Figure 3 Effects of oral EVOO administration (300 μl) and K₂Cr₂O₇ (0.4 mg/kg bw/day) on Phagocytic% (A), Phagocytic index (B), Nitric oxide level (C) and Lysozyme activity (D). Each bars carrying different letters were significantly different (P′ < 0.05) (Mean ± SE, n = 6).

Figure 4 Photomicrographs of the H&E paraffin stained sections of; (A) K₂Cr₂O₇ treated rat spleen (E. Higher magnification), (B) K₂Cr₂O₇ treated and EVOO-administered rat spleen (F. Higher magnification), (C) EVOO administered rat spleen (G. Higher magnification), (D) Control rat spleen (H. Higher magnification) (Bar = 100 μm).

Figure 5 Immunohistochemical staining of CD3 in rat spleen of (A) K₂Cr₂O₇ treated group (B) K₂Cr₂O₇ treated and EVOO-administered group (C) EVOO administered group (D) Control group, IHC (Bar = 100 μm).

Figure 6 Immunohistochemical staining of B220 in rat spleen of (A) K₂Cr₂O₇ treated group (B) K₂Cr₂O₇ treated and EVOO-administered group (C) EVOO administered group (D) Control group, IHC (Bar = 100 μm).