Pathogenicity testing and antimicrobial susceptibility of Helicobacter pullorum isolates from chicken origin

Peer review under responsibility of Faculty of Veterinary Medicine, Cairo University.

Figures & data

Fig. 1 Showing number of deaths of experimentally infected chicks with H. pullorum infection per day post infection.

Table 1 Showing results of experimental infection of one-day old chicks with H. pullorum isolates.

Group	Inoculated agent	No. of chicks	Rout of inoculation	No. of deaths/day post infection						Total no. of deaths	No. of survivors	Mortality percentage (%)
				4th day	5th day	6th day	7th day	8th day	9th day
Experimental	H. pullorum isolates	39	Oral	4	3	2	2	1	1	13	26	33.3
Control	Sterile BHI broth	13	Oral	–	–	–	–	–	–	–	13	00.0

Fig. 2 PCR positive reaction from H. pullorum isolates to 16S-rRNA gene. Lane M: 1 Kb plus DNA ladder.

Fig. 3 Showing percentage of resistant isolates of H. pullorum for each antibacterial drug used in the experiment.

Table 2 Showing results of susceptibility of 8 chicken isolates of H. pullorum to antimicrobial agents.

Antibiotic	No. of isolates with MIC (μg/ml) of:											Break-point of drug resistance	No. of resistant isolates	% of resistant isolates (%)
	0.25	0.5	1	2	4	8	16	32	64	128	256
Ciprofloxacin	–	–	–	–	–	2	1	5	–	–	–	⩾4	8	100
Gentamycin	–	–	–	–	–	–	–	4	4	–	–	⩾16	8	100
Colistin	–	–	–	–	–	–	4	4	–	–	–	⩾32	–	0
Tetracycline	–	–	–	–	–	–	1	4	3	–	–	⩾16	7	87.5
Erythromycin	–	–	–	–	–	–	2	2	4	–	–	⩾8	8	100
Ampicillin	–	–	–	–	–	2	–	6	–	–	–	⩾32	–	0