Evaluation of oxidative stress in caprine bronchoalveolar lavage fluid of pneumonic and normal lungs

Peer review under responsibility of Faculty of Veterinary Medicine, Cairo University.

Figures & data

Fig. 1 Distribution of bronchoalveolar lavage fluid anti-oxidants levels from normal and pneumonic goats. Br Pn- Bronchopneumonia; Br In- Bronchointerstitial pneumonia; Int pn- Interstitial pneumonia; Gran pn- Granulomatous pneumonia.TP- Total Protein; GPx- Glutathione peroxidase; GSH- Reduced glutathione; GST- Glutathione transferase; SOD- Superoxide dismutase.

Fig. 2 Distribution of bronchoalveolar lavage fluid pro-oxidants levels from normal and pneumonic goats. Br Pn- Bronchopneumonia; Br In- Bronchointerstitial pneumonia; Int pn- Interstitial pneumonia; Gran pn- Granulomatous pneumonia; H₂O₂- Hydrogen peroxide; MDA- Malondialdehyde; MPO- Myeloperoxidase.

Table 1 The total protein, anti-oxidants and pro-oxidants values in bronchoalveolar lavage fluid from normal and pneumonic goats.

Lung lesion	TP mg/mL	GPX units mg^−1	GSH µg mL^−1	GST unit mg^−1 p	SOD unit mg^−1 p	H2O2 µmol mg^−1 p	MDA µmol/mL	MPO µmol/min
Normal	9.56 ± 0.7^a	24.1 ± 1.5^a	9.9 ± 0.3^a	8.0 ± 1.1^a	4.1 ± 0.3^a	3.6 ± 0.5^a	2.3 ± 0.1^a	2.0 ± 0.2^a
Oedema	11.6 ± 1.1^a	21.4 ± 1.7^a	9.4 ± 0.2^a	6.5 ± 1.0^b	3.6 ± 0.3^a	5.0 ± 0.4^b	2.1 ± 0.1^a	16.0 ± 0.1^b
Br pn	16.7 ± 1.7^b	18.9 ± 1.4^b	9.7 ± 0.1^a	7.0 ± 0.8^a	3.2 ± 0.2^a	4.9 ± 0.3^b	2.3 ± 0.1^a	20.0 ± 0.1^b
Br In Pn	13.5 ± 1.7^a	20.1 ± 1.8^a	9.5 ± 0.1^a	6.3 ± 0.9^b	3.4 ± 0.3^a	5.0 ± 0.6^b	4.43 ± 0.1^b	30.0 ± 0.2^c
Int pn	15.4 ± 3.5^b	22.3 ± 2.7^a	9.9 ± 0.3^a	8.6 ± 1.1^a	3.7 ± 0.4^a	5.2 ± 0.4^b	2.2 ± 0.1^a	10.0 ± 0.1^b
Gran pn	15.2 ± 4.8^b	20.4 ± 4.9 ^a	10.2 ± 0.4^a	8.1 ± 3.3^a	3.4 ± 0.8^a	5.6 ± 0.7^b	3.0 ± 0.1^a	11.0 ± 0.1^b

Values with different superscripts are significantly different within column.

Br Pn- Bronchopneumonia; Br In- Bronchointerstitial pneumonia; Int pn- Interstitial pneumonia; Gran pn- Granulomatous pneumonia.

TP- Total Protein; GPx- Glutathione peroxidase; GSH- Reduced glutathione; GST- Glutathione transferase; H₂O₂- Hydrogen peroxide; MDA- Malondialdehyde; MPO- Myeloperoxidase; SOD- Superoxide dismutase.