Effect of oviduct and follicular fluids on ram sperm capacitation and acrosome reaction in vitro

Peer review under responsibility of Faculty of Veterinary Medicine, Cairo University.

Figures & data

Table 1 Effect of different oviduct fluid (OF) concentrations (µL/mL) and incubation time (h) on motility of ram spermatozoa (Means ± SE).

Concentrations of OF	Incubation time (h)
	0	1	2	3	4
Control	83.0 ± 0.81^Aa	80.0 ± 0.00 ^Aa	78.0 ± 0.81^Aa	69.0 ± 0.83^Ab	32.5 ± 1.69^Ac
10	83.5 ± 0.76^Aa	79.5 ± 1.16^Aa	79.0 ± 1.24^Aa	69.5 ± 1.57^ABb	52.0 ± 4.54^ABc
20	81.5 ± 1.30^Aa	76.0 ± 1.45^ABab	71.5 ± 0.76^Bb	62.0 ± 2.00^Cc	36.0 ± 3.23^CDd
50	81.0 ± 0.66^Aa	80.5 ± 1.38^Aa	79.5 ± 1.16^Aa	72.0 ± 1.79^ABb	57.5 ± 3.18^Dc
75	79.5 ± 1.38^Aa	73.5 ± 2.24^Bb	66.5 ± 1.30^Cc	65.5 ± 1.38^BCc	44.5 ± 2.03^BCd

Means with different alphabetical superscripts in the same columns A, B, C, D and different rows a, b, c are significant at least at P < .05.

Table 2 Effect of different follicular fluid (FF) concentrations (µL/mL) and incubation time (h) on motility of ram spermatozoa (Mean ± SE).

Concentrations of FF	Incubation time
	0	1	2	3	4
Control	79.5 ± 0.50^Ca	77.0 ± 0.81^Aa	75.0 ± 1.49^Aa	64.5 ± 2.62^Bb	41.5 ± 3.16^Bc
10	84.5 ± 0.50^Aa	80.0 ± 1.05^Aab	75.0 ± 1.05^Ab	78.5 ± 2.47^Aab	57.0 ± 4.36^Ac
20	84.0 ± 0.66^Aa	80.0 ± 1.05^Aab	75.0 ± 1.49^Ab	73.0 ± 2.49^ABb	52.0 ± 4.54^ABc
40	84.5 ± 0.50^Aa	79.0 ± 1.24^Ab	71.5 ± 1.50^Ac	75.0 ± 2.68^ABbc	45.5 ± 1.38^ABd
50	84.0 ± 0.66^Aa	80.0 ± 1.05^Aab	76.5 ± 1.83^Abc	73.5 ± 2.11^ABc	52.5 ± 1.53^ABd
100	83.0 ± 0.81^Aa	76.0 ± 1.24^Ab	73.0 ± 1.33^Ab	65.0 ± 1.49^Bc	40.0 ± 2.58^Bd
150	80.0 ± 1.05^BCa	79.0 ± 1.24^Aa	70.0 ± 1.82^Ab	74.5 ± 3.37^Bab	44.5 ± 2.40^Bc
200	82.0 ± 0.81^ABa	75.5 ± 1.16^Aab	74.0 ± 1.24^Aab	71.0 ± 4.13^ABb	39.5 ± 3.37^Bc

Means with different alphabetical superscripts in the same columns A, B, C, D and different rows a, b, c are significant at least at P < .05.

Table 3 Effect of different oviduct fluid (OF) concentrations (µL/mL) and incubation time (Hours) on hyperactivity of ram spermatozoa (Means ± SE).

Concentrations of OF	Incubation time (h)
	0	1	2	3	4
Control	0.0 ± 0.00^Ca	0.0 ± 0.00^Da	0.0 ± 0.00^Da	1.0 ± 0.00^Ca	1.0 ± 0.00^Da
10	0.0 ± 0.00^Cd	1.4 ± 0.26^Cc	2.6 ± 0.26^Cb	3.6 ± 0.26^ABa	2.2 ± 0.24^BCb
20	0.6 ± 0.26^BCc	2.2 ± 0.38^BCb	4.0 ± 0.29^Ba	3.8 ± 0.24^Aa	2.4 ± 0.16^ABb
50	2.0 ± 0.20^Ad	4.4 ± 0.44^Ab	5.6 ± 0.16^Aa	4.2 ± 0.24^Ab	2.8 ± 0.13^Ac
75	0.8 ± 0.24^Bd	2.6 ± 0.16^Bb	4.0 ± 0.20^Ba	3.0 ± 0.20^Bb	1.8 ± 0.13^Cc

Means with different alphabetical superscripts in the same columns A,B,C,D and different rows a,b,c are significant at least at P < .05.

Table 4 Effect of different follicular fluid concentrations (µL/mL) and incubation time (h) on Hyperactivity of ram spermatozoa (Mean ± SE).

Concentrations of FF	Incubation time (h)
	0	1	2	3	4
Control	0.0 ± 0.00^Ac	0.0 ± 0.00^Cc	0.0 ± 0.00^Bc	1.4 ± 0.26^Ba	0.6 ± 0.16^Bb
10	0.0 ± 0.00^Ac	3.0 ± 0.29^Bb	5.6 ± 0.49^Aa	5.8 ± 0.61^Aa	3.2 ± 0.24^Ab
20	0.0 ± 0.00^Ac	2.6 ± 0.33^Bb	5.8 ± 0.67^Aa	5.0 ± 0.59^Aa	3.0 ± 0.42^Ab
40	0.2 ± 0.13^Ac	3.0 ± 0.59^Bb	5.2 ± 0.24^Aa	5.8 ± 0.61^Aa	3.4 ± 0.16^Ab
50	0.4 ± 0.26^Ac	4.6 ± 0.39^Aa	5.4 ± 0.16^Aa	4.8 ± 0.57^Aa	3.0 ± 0.20^Ab
100	0.2 ± 0.13^Ad	3.2 ± 0.48^Bbc	5.0 ± 0.42^Aa	3.8 ± 0.13^Ab	2.6 ± 0.16^Ac
150	0.4 ± 0.26^Ac	3.8 ± 0.43^ABb	6.0 ± 0.42^Aa	5.0 ± 0.51^Aa	3.2 ± 0.13^Ab
200	0.8 ± 0.32^Ac	2.4 ± 0.33^Bb	6.2 ± 0.74^Aa	5.8 ± 0.48^Aa	2.8 ± 0.24^Ab

Means with different alphabetical superscripts in the same columns A, B, C, D and different rows a, b, c are significant at least at P < .05.

Table 5 Effect of different oviduct fluid concentrations (µL/mL) and incubation time (h) on total acrosome reaction of ram spermatozoa (Mean ± SE).

Concentrations of OF	Incubation time (h)
	0	1	2	3	4
Control	0.0 ± 0.0^Ac	0.0 ± 0.0^Cc	0.0 ± 0.0^Ec	8.9 ± 0.67^Eb	15.4 ± 2.56^Da
10	0.0 ± 0.0^Ad	0.0 ± 0.00^Cd	8.2 ± 1.0^Dc	29.4 ± 3.21^Db	41.7 ± 1.93^Ca
20	0.0 ± 0.0^Ad	7.0 ± 0.86^Bc	26.1 ± 3.19^Cb	51.1 ± 2.53^Ca	53.8 ± 1.62^Ba
50	0.0 ± 0.0^Ad	0.0 ± 0.00^Cd	84.1 ± 2.0^Ab	92.5 ± 1.55^Aa	71.4 ± 2.63^Ac
75	0.0 ± 0.0^Ae	11.6 ± 1.07^Ad	37.0 ± 2.64^Bc	70.7 ± 2.58^Ba	54.3 ± 4.48^Bb

Means with different alphabetical superscripts in the same columns A, B, C, D and different rows a, b, c are significant at least at P < .05.

Table 6 Effect of different follicular fluid concentrations (µL/mL) and incubation time (h) on total acrosome reaction of ram spermatozoa (Mean ± SE).

Concentrations of FF	Incubation time (h)
	0	1	2	3	4
Control	0.0 ± 0.00^Bc	0.0 ± 0.00^Ec	7.0 ± 1.18^Fb	16.7 ± 0.95^Ba	17.2 ± 1.95^Da
10	0.0 ± 0.00^Bd	4.1 ± 0.68^DEd	24.7 ± 1.93^Ec	68.0 ± 4.69^Aa	58.3 ± 2.76^Cb
20	0.0 ± 0.00^Bd	6.4 ± 1.03^Dd	36.1 ± 1.66^Dc	72.50 ± 4.31^Aa	63.1 ± 3.11^BCb
40	0.0 ± 0.00^Bd	34.6 ± 2.12^Ac	62.4 ± 3.02^Ab	74.8 ± 2.98^Aa	80.0 ± 3.53^Aa
50	0.0 ± 0.00^Bd	17.6 ± 1.87^BCc	30.9 ± 1.84^DEb	66.1 ± 2.11^Aa	63.1 ± 2.75^BCa
100	0.0 ± 0.00^Bd	21.6 ± 2.45^Bc	46.4 ± 3.01^BCb	77.3 ± 4.02^Aa	75.3 ± 2.81^Aa
150	1.6 ± 0.33^Bd	14.0 ± 1.32^Cc	39.1 ± 3.67^CDb	69.3 ± 2.55^Aa	72.6 ± 2.86^ABa
200	11.1 ± 1.29^Ae	32.2 ± 1.95^Ad	50.1 ± 4.03^Bc	71.4 ± 2.37^Aa	62.2 ± 4.01^BCb

Means with different alphabetical superscripts in the same columns A, B, C, D and different rows a, b, c are significant at least at P < .05.

Table 7 Effect of best concentration of oviduct fluid (50 µL/mL) and follicular fluid (40 µL/mL) on in vitro fertilization of ovine oocytes.

Capacitating Compounds	Number of matured oocytes	Penetration rate (%)	Fertilization rate (%)
Control	90	40/90(44.4)^b	15/40(37.5)^b
Oviduct fluid	100	74/100(74.0)^a	55/74(74.3)^a
Follicular fluid	100	80/100(80.0)^a	60/80(75.0)^a

Means with different superscripts in the same columns a, b are significant at least at P < .05