The effect of albumin administration on renal dysfunction after experimental surgical obstructive jaundice in male rats

Peer review under responsibility of Taibah University

Figures & data

Table 1 Haematocrit value and ratios of the liver and kidney-to-body weight in the different experimental groups.

Parameters	Groups
	Sham/untreated Group I	Sham/treated Group II	BDL/untreated Group III	BDL/treated Group IV
Haematocrit value (%)	42 ± 1.02	40 ± 1.14	39 ± 2.04	38 ± 1.01
Liver to body weight ratio (%)	3.15 ± 0.25	3.09 ± 0.35	5.10 ± 0.55^*	4.15 ± 0.33^*
Kidney to body weight ratio (%)	0.29 ± 0.05	0.32 ± 0.03	0.41 ± 0.02^*	0.39 ± 0.03^*

Values are the means ± SEM.

* Significance compared to the sham control groups.

Table 2 Kidney and liver function parameters in the different experimental groups.

Parameters	Groups
	Sham/untreated Group I	Sham/treated Group II	BDL/untreated Group III	BDL/treated Group IV
T. BIL (mg/dl)	0.32 ± 0.02	0.25 ± 0.05	10.17 ± 2.02^*	9.11 ± 1.42^*
D. BIL (mg/dl)	0.08 ± 0.009	0.05 ± 0.009	5.11 ± 1.02^*	6.09 ± 1.12^*
AST (IU/l)	57.99 ± 6.34	43.46 ± 4.56	722.22 ± 92.1^*	381 ± 44.6^*^,^¥
ALT (IU/l)	32.47 ± 5.92	36.22 ± 4.61	208.28 ± 45.2^*	122.56 ± 41.8^*^,^¥
ALP (IU/l)	75.78 ± 8.6	66.62 ± 6.16	361.98 ± 71.3^*	218.27 ± 41.5^*^,^¥
Albumin (gm/dl)	2.97 ± 0.13	3.57 ± 0.36	1.51 ± 0.83^*	2.78 ± 0.59^¥
BUN (mg/dl)	40.61 ± 6.16	35.37 ± 5.22	32.44 ± 7.03	39.16 ± 4.38
Creatinine (mg/dl)	0.25 ± 0.09	0.31 ± 0.08	1.21 ± 0.15^*	0.79 ± 0.08^*^,^¥

Values are the means ± SEM.

* Significance compared to the sham control groups.

¥ Significance between group III and IV.

Fig. 1 Levels of renal tissue MDA in different experimental groups. The results are the means and standard errors. ^*Significance compared to the sham control groups.

Fig. 2 Tissue glutathione levels in different experimental groups. The results are the means and standard errors. ^*Significance compared to the sham control groups. ^¥Significance between groups III and IV.

Fig. 3 Tissue catalase activity in the different experimental groups. The results are the means and standard errors. ^*Significance compared to the sham control groups. ^¥Significance between groups III and IV.

Fig. 4 Tissue NO levels in the different experimental groups. The results are the means and standard errors. ^*Significance compared to the sham control groups. ^¥Significance between groups III and IV.

Fig. 5 (a) Photomicrograph of a liver of a sham untreated albino rat showing normal hepatic lobulation and normal hepatic plates (H&E 400×). (b) Photomicrograph of a liver of a sham treated albino rat showing normal hepatic plates; note hepatocytes with normal cytoplasmic density and normal nuclei (H&E 1000×). (c) Photomicrograph of a liver of an albino rat 4 weeks after BDL showing severe hepatic fibrosis (H&E 400×). (d) Photomicrograph of a liver of an albino rats 4 weeks after BDL showing the hepatic lobules replaced with collagenic fibrosis (c) and the newly formed bile ducts (D). (Crossmon's trichrome 400×). (e) Photomicrograph of a liver from a BDL/Albumin treated albino rat showing degenerated areas with inflammatory cells infiltration (D) and some normal areas (n) (H&E 200×). (f) Photomicrograph of a liver from a BDL/Albumin treated albino rat showing some normal hepatocytes (arrow) (H&E 400×).

Fig. 6 (a) Photomicrograph of a renal medulla of a sham untreated albino rat showing normal thin segments of the loop of Henle (arrow) and collecting tubules (c) (H&E 320×). (b) Photomicrograph of a kidney of a sham treated albino rat showing normal thick segments of the loop of Henle (arrow) (H&E 200×). (c) Photomicrograph of a kidney from a BDL/Albumin treated albino rat showing some recovery in some renal medulla with decrease the interlobular fluid and some focal area of lymphocytic infiltration (arrow). (H&E 400×). (d) Photomicrograph of a kidney from an albino rat 4 weeks after BDL showing severe renal damage. Note the swelling of renal corpuscles (R), haemorrhage (arrow) and eosinophilic fluid (arrow head) of granulose cells (arrow head) (H&E 100×). (e) Photomicrograph of a kidney from an albino rat 4 weeks after BDL showing swelling in the renal corpuscles (R) with vacuolated and damaged epithelium of the tubules (arrow), some of which appeared to be ruptured (arrow head) (H&E 400×). (f) Photomicrograph of a kidney from a BDL/Albumin treated albino rat showing recovery in some renal corpuscles (R) and renal tubules (arrow head). Note the infiltration of the inflammatory cell (arrow) and wide tubules (w) (H&E 400×).