Toxicity and stability of sodium cyanide in fresh water fish Nile tilapia

Peer review under responsibility of National Water Research Center.

Figures & data

Fig. 1 Determination of LC₅₀ of sodium cyanide in Nile tilapia.

Fig. 2 The mean concentration of Na⁺/K⁺ ATPase (μmol Pi liberated/mg protein/h) within the gill filaments, liver and muscles of fish after 1, 7, 14, 21 and 28 days of exposure.

Table 2 The mean activities of Na⁺/K⁺, Mg²⁺ and Ca²⁺-ATPase (μmol Pi liberated/mg protein/h) in stored frozen different tissues of tilapia in different times.

Time	Control	24 h	7 days	14 days	21 days	28 days
Na^+/K^+-ATPase (μmol Pi liberated/mg protein/h) ± S.D.
Gills	13.51 ± 0.61	15.01 ± 0.37	10.76 ± 0.62	7.45 ± 0.41	6.49 ± 0.74	6.12 ± 0.35
Liver	11.15 ± 0.53	13.45 ± 0.54	9.72 ± 0.46	7.86 ± 0.69	6.95 ± 0.75	5.44 ± 0.75
Muscles	8.37 ± 0.57	10.02 ± 0.41	7.47 ± 0.67	5.68 ± 0.49	4.53 ± 0.46	4.00 ± 0.29
Mg^2+-ATPase (μmol Pi liberated/mg protein/h) ± S.D.
Gills	8.91 ± 0.05	9.65 ± 0.08	6.73 ± 0.13	5.42 ± 0.06	5.03 ± 0.21	4.32 ± 0.41
Liver	3.63 ± 0.22	5.83 ± 0.19	2.52 ± 0.06	1.92 ± 0.04	1.64 ± 0.06	1.00 ± 0.04
Muscles	2.14 ± 0.09	4.49 ± 0.10	1.24 ± 0.08	0.91 ± 0.02	0.88 ± 0.02	0.53 ± 0.02
Ca^2+-ATPase (μmol Pi liberated/mg protein/h) ± S.D.
Gills	8.13 ± 0.07	9.13 ± 0.25	7.18 ± 0.13	6.51 ± 0.08	5.39 ± 0.09	4.21 ± 0.13
Liver	5.82 ± 0.57	7.35 ± 0.44	4.32 ± 0.43	3.97 ± 0.31	2.98 ± 0.24	2.02 ± 0.21
Muscles	4.77 ± 0.16	6.41 ± 0.25	3.47 ± 0.10	2.75 ± 0.12	1.96 ± 0.12	1.24 ± 0.08

Fig. 3 The mean concentration of Mg²⁺ ATPase (μmol Pi liberated/mg protein/h) within the gill filaments, liver and muscles of fish after 1, 7, 14, 21 and 28 days of exposure.

Fig. 4 The mean concentration of Ca²⁺ ATPase (μmol Pi liberated/mg protein/h) within the gill filaments, liver and muscles of fish after 1, 7, 14, 21 and 28 days of exposure.

Table 1 Mean concentration of cyanide (mg/kg) and its percentage (%) in stored frozen different tissues of tilapia in different times.

Time	Gills		Liver		Muscles		Blood
	%	Mean ± S.D. (mg/kg)	%	Mean ± S.D. (mg/kg)	%	Mean ± S.D. (mg/kg)	%	Mean ± S.D. (mg/kg)
Control	0	0.0	0	0.0	0	0.0	0%	0.0
20 min	89	0.115 ± 0.011	73	0.094 ± 0.04	83	0.107 ± 0.01	91.5	0.118 ± 0.05
1 h	83	0.107 ± 0.01	69	0.089 ± 0.03	72	0.093 ± 0.03	86	0.111 ± 0.02
12 h	79	0.102 ± 0.02	56.6	0.073 ± 0.01	56.6	0.073 ± 0.04	63.6	0.082 ± 0.02
24 h	68.9	0.089 ± 0.05	46.2	0.059 ± 0.02	43.7	0.056 ± 0.01	56.6	0.073 ± 0.03
48 h	44.8	0.058 ± 0.04	0.0	0.0	0.0	0.0	45.7	0.059 ± 0.03
72 h	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0
96 h	0.0	0.0	0.0	0.0	0.0	0.0	0.0	0.0