Identification and characterization of a novel incompatibility group X3 plasmid carrying bla_NDM-1 in Enterobacteriaceae isolates with epidemiological links to multiple geographical areas in China

Figures & data

Table 1 Patient demographics, bacterial strains and features of plasmids carrying bla_NDM-1

Collection date	Strain NO	Sex/age	Place of medicalcare abroad	Bacterial species	Resistance patterns^b	MLST	Plasmidreplicon type	Plasmidsize (kb)
Dec 2010	172924	F/54	India	E. coli	Gen, Amk, Sxt, Tet, Cip, Chl	ST101	FIIY-FIBS^c	110
March 2011	CRE851	M/60	Guangzhou	C. freundii	Gen, Amk, Sxt, Cip	—	X3	50
July 2011	CRE379	F/1^a	Hunan	E. aerogenes	Fot	—	X3	50
Aug 2011	CRE380	F/1^a	Hunan	K. pneumoniae	Gen, Sxt, Tet, Cip, Chl	ST483	X3	50
Aug 2011	CRE396	F/1^a	Hunan	E. coli	Sxt, Tet, Cip, Chl	ST744	X3	50
Aug 2011	CRE397	F/26^a	Hunan	E. coli	Sxt, Tet, Cip, Chl	ST744	X3	50
Nov 2011	CRE866	M/74	Fujian	E. coli	Gen, Amk, Sxt, Tet, Cip, Chl, Fot	ST101	A/C2	140
Feb 2012	CRE727	M/82	Haifeng	E. cloacae	Gen, Amk, Sxt, Tet, Cip, Chl, Fot	—	X3	50
May 2012	CRE843	M/1	Dongguan	K. pneumoniae	Gen, Sxt, Tet, Cip	ST476	X3	50

a Strains CRE79, CRE380 and CRE396 were recovered from the same patient. The two patients (F/1 and F/26) were of the same family.

b For the following drugs, Gen, gentamicin; Amk, amikacin; Sxt, cotrimoxazole; Tet, tetracycline; Cip, ciprofloxacin; Chl, chloramphenicol; Fot, fosfomycin.

c Positive for both FII_Y (allele Y3) and FIB_S (Salmonella FIB) replicons.³⁰

Figure 1 Restriction analysis of IncX3 plasmids carrying bla_NDM-1. Plasmids were digested with (A) EcoRI and (B) PstI and separated by electrophoresis in 1% agarose. M, GeneRulerTM DNA ladder. The labels above each lane show the strain number, bacterial species origin (EA, E. aerogenes; KP, K. pneumoniae; EC, E. coli; EO, E. cloacae; CF, C. freundii) and the geographic source of importation (HN, Hunan; HF, Haifeng; DG, Dongguan; GZ, Guangzhou).

Figure 2 Comparative analysis of (A) linear plasmid maps for three IncX3 plasmids, pEC14_35, pIncX-SHV, pNDM-HN380 and two bla_NDM-1-carrying transposon sequences in pNDM-BJ01 and A. baumannii strain 161/07; (B) sequences downstream of insE and (C) sequences upstream of the ISAba125 in the 5′ end of bla_NDM-1. The function blocks of the plasmids are indicated above the linear maps. The lengths of the ORFs are drawn in proportion to the size of the ORFs. Homologous ORFs in the plasmid maps are represented in the same colour. Direct repeats and mobile elements are labelled in blue and red, respectively. (B, C) Consensus regions in the aligned sequences of pNDM-HN380, pNDM-BJ01 and 161/07 are marked with asterisk. The sequences identical in pNDM-HN380 and pNDM-BJ01 are coloured green. The ORFs are indicated by grey shading and the arrow next to the label indicates the ORF orientation. The accession numbers were: pEC14_35 (JN935899), pIncX-SHV (JN247852), pNDM-HN380 (JX104760), pNDM-BJ01 (JQ001791) and Acinetobacter baumannii strain 161/07 (HQ857107).

Villa L, Garcia-Fernandez A, Fortini D, Carattoli A.Replicon sequence typing of IncF plasmids carrying virulence and resistance determinants. J Antimicrob Chemother2010;65: 2518–2529.

 PubMed Web of Science ®Google Scholar